Development and optimization of perfusion process for mammalian cell culture.

Author: Qiongqiong ZHANG ¹ ; Mingyue FANG ² ; Junjie LI ² ; Rongyue CAO ¹
Author Information

1. Life Science and Technology College, China Pharmaceutical University, Nanjing 211100, Jiangsu, China.
2. Cell Culture Process Development Department, Wuxi Biologics, Shanghai 200131, China.
Publication Type:Journal Article
Keywords: cell specific perfusion rate; filter clogging; hollow fiber filter; perfusion culture
MeSH: Animals; Batch Cell Culture Techniques; trends; Bioreactors; standards; CHO Cells; Cricetulus; Mammals; Perfusion
From: Chinese Journal of Biotechnology 2020;36(6):1041-1050
CountryChina
Language:Chinese
Abstract: In recent years, the demand of biologics has increased rapidly. Cell culture process with perfusion mode has become more and more popular due to its high productivity, good quality and high efficiency. In this paper, the unique operation and the details of process optimization for perfusion culture mode are discussed by comparing with traditional batch culture process. Meanwhile, the progress and strategies in the development and optimization of perfusion culture process in recent years are summarized to provide reference for the future development of mammalian cell perfusion culture technology.