Progress in fluorescent visualization techniques for neurotransmitter detection.

Author: Qiao HU ¹ ; Yuxin SHI ¹ ; Xiaoling YANG ¹ ; Jing ZHANG ¹ ; Bo LI ¹ ; Xiaoling LIAO ¹ ; Xue LIU ¹
Author Information

1. Chongqing Key Laboratory of Nano/Micro Composite Materials and Devices, Chongqing University of Science and Technology, Chongqing 401331, China.
Publication Type:Journal Article
Keywords: fluorescence resonance energy transfer; fluorescent protein; neurotransmitter; visualization
MeSH: Animals; Biosensing Techniques; instrumentation; trends; Fluorescence; Humans; Neurotransmitter Agents; metabolism
From: Chinese Journal of Biotechnology 2020;36(6):1051-1059
CountryChina
Language:Chinese
Abstract: Neurotransmitters play an important role in nervous system. Temporal and spatial changes of neurotransmitter distribution are crucial to information processing in neural networks. Biosensors that can visually monitor neurotransmitters are one of the vital tools to explore a variety of physiological and pathological activities. This article reviews recent advances in monitoring neurotransmitters with high temporal and spatial resolution, and introduces the latest fluorescent imaging methods for typical neurotransmitters, including glutamate, dopamine, γ-aminobutyric acid and acetylcholine. The article also summarizes the basic principles, advantages and disadvantages of various visually detection methods, and provides systematic suggestions for designing neurotransmitter sensors with high temporal and spatial resolution.