Advances in the research and applications of orange fluorescent protein.
- Author:
Wen PENG
1
;
Peimin HE
1
;
Dingji SHI
1
;
Rui JIA
1
Author Information
- Publication Type:Journal Article
- Keywords: biosensor; fluorescence resonance energy transfer; orange fluorescent protein; reporter protein
- MeSH: Biosensing Techniques; trends; Fluorescence Resonance Energy Transfer; Luminescent Proteins; metabolism; Research; trends
- From: Chinese Journal of Biotechnology 2020;36(6):1060-1068
- CountryChina
- Language:Chinese
- Abstract: Fluorescent proteins can be used as probes to investigate intercellular molecular interactions and trace the pathway of specific metabolites, thus providing a detailed and accurate description of various metabolic processes and cellular pathways in living cells. Nowadays, the existing fluorescent proteins cover almost all spectral bands from ultraviolet to far-red. These fluorescent proteins have been applied in many fields of bioscience with the help of high-resolution microscopy, making great contributions to the development of biology. It is generally agreed that orange fluorescent proteins refer to the fluorescent proteins at the spectral range of 540-570 nm. In recent years, researches on orange fluorescent proteins have made great progress, and they have been widely applied in the field of biology and medicine as reporter protein and fluorescence resonance energy transfer as fluorescent receptor. This paper reviews the studies in the field of orange fluorescent proteins over the last 15 years, with the special focus on the development and application of orange fluorescent proteins to provide the basis for the future studies.
