The 40-91 aa sequence of porcine epidemic diarrhea virus ORF3 protein is the key structural domain controlling its location in cytoplasm.

Bingqing CHEN; Mei SHEN; Fusheng SI; ShiJuan DONG; RuiSong YU; ChunFang XIE; Zhen LI

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The 40-91 aa sequence of porcine epidemic diarrhea virus ORF3 protein is the key structural domain controlling its location in cytoplasm.

Author: Bingqing CHEN ¹ ; Mei SHEN ² ; Fusheng SI ¹ ; ShiJuan DONG ¹ ; RuiSong YU ¹ ; ChunFang XIE ¹ ; Zhen LI ¹
Author Information

1. Institute of Animal Husbandry & Veterinary Science, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
2. College of Fisheries and Life Science, shanghai Ocean University, Shanghai 201306, China.
Publication Type:Journal Article
Keywords: ORF3 protein; cytoplasmic location; location signal; porcine epidemic diarrhea virus; transmembrane domain
MeSH: Amino Acid Sequence; Animals; Chlorocebus aethiops; Coronavirus Infections; virology; Cytoplasm; virology; Porcine epidemic diarrhea virus; genetics; Protein Domains; Swine; Vero Cells; Viral Proteins; chemistry; metabolism
From: Chinese Journal of Biotechnology 2020;36(6):1113-1125
CountryChina
Language:Chinese
Abstract: ORF3 protein, the single accessory protein encoded by porcine epidemic diarrhea virus (PEDV), is related to viral pathogenicity. In order to determine the cytoplasmic location signal of PEDV ORF3, we constructed a series of recombinant plasmids carrying full-length or truncated segments of PEDV DR13 ORF3 protein. When the acquired plasmids were transfected into Vero cells, expression and distribution of the EGFP-fused full-length ORF3 protein and its truncated forms in the cells were observed by laser confocal microscopy. The results showed that ORF3 protein or their truncated forms containing 40-91 aa segment including two transmembrane domains were localized in the cytoplasm, whereas ORF3 truncated peptides without the 40-91 aa segment were distributed in the whole cell (in both cytoplasm and nucleus). This suggests that the 40-91 aa is the key structural domain determining cytoplasmic location of PEDV ORF3 protein. The discovery provides reference for further clarifying intracellular transport and biological function of PEDV ORF3 protein.