Numerical simulation and optimization of impeller combination used in stirred bioreactor.
- Author:
Ning DING
1
;
Chao LI
1
;
Li BAI
1
;
Meijin GUO
1
;
Yingping ZHUANG
1
;
Siliang ZHANG
1
Author Information
- Publication Type:Journal Article
- Keywords: CHO culture; bioreactor; impeller combination; numerical simulation
- MeSH: Animals; Batch Cell Culture Techniques; Bioreactors; standards; CHO Cells; Cell Count; Computer Simulation; Cricetinae; Cricetulus; Industrial Microbiology; instrumentation; methods
- From: Chinese Journal of Biotechnology 2020;36(6):1209-1215
- CountryChina
- Language:Chinese
- Abstract: Bioreactors have been central in monoclonal antibodies and vaccines manufacturing by mammalian cells in suspension culture. Numerical simulation of five impeller combinations in a stirred bioreactor was conducted, and characteristics of velocity vectors, distributions of gas hold-up, distributions of shear rate in the bioreactor using 5 impeller combinations were numerically elucidated. In addition, genetically engineered CHO cells were cultivated in bioreactor installed with 5 different impeller combinations in fed-batch culture mode. The cell growth and antibody level were directly related to the maximum shear rate in the bioreactor, and the highest viable cell density and the peak antibody level were achieved in FBMI3 impeller combination, indicating that CHO cells are sensitive to shear force produced by impeller movement when cells were cultivated in bioreactor at large scale, and the maximum shear rate would play key roles in scaling-up of bioreactor at industrial scale.
