ISA 61 VG adjuvant enhances protective immune response of Listeria monocytogenes inactivated vaccine.

Tengfei Zhu; Fanzeng Meng; Hao Yao; Yuting Wang; Xin'an Jiao; Yuelan Yin

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ISA 61 VG adjuvant enhances protective immune response of Listeria monocytogenes inactivated vaccine.

Author: Tengfei ZHU ¹ ; Fanzeng MENG ¹ ; Hao YAO ¹ ; Yuting WANG ¹ ; Xin'an JIAO ¹ ; Yuelan YIN ¹
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1. Yangzhou University, College of Bioscience and Biotechnology/Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses/Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China/Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou 225009, Jiangsu, China.
Publication Type:Journal Article
Keywords: Listeria monocytogenes; adjuvant; inactivated vaccine; safety; vaccine potency
MeSH: Adjuvants, Immunologic; pharmacology; Animals; Hemolysin Proteins; immunology; pharmacology; Immunity, Cellular; drug effects; Listeria monocytogenes; immunology; Listeriosis; prevention & control; Mice; Mice, Inbred BALB C; Vaccines, Inactivated; immunology
From: Chinese Journal of Biotechnology 2020;36(7):1378-1385
CountryChina
Language:Chinese
Abstract: Listeria monocytogenes (Lm) is zoonotic pathogen that can cause listeriosis, and vaccine is one of the effective methods to prevent this pathogen infection. In this study, we developed a novel vaccine that is a mixture of inactivated bacteria and Montanide™ ISA 61 VG, a mineral oil adjuvant, and evaluated the safety and immune response characteristics of this vaccine. The mice immunized with the ISA 61 VG adjuvant had high safety, and it could induce significantly higher titer of anti-listeriolysin O (LLO) antibody and higher value of IgG2a/IgG1 ratio compared with the group without the adjuvant. In particular, it could provide 100% immune protection against lethal doses of Lm challenge in mice. In summary, ISA 61VG adjuvant significantly enhanced the ability of inactivated listeria vaccine to induce humoral and cellular immune responses, thereby enhanced the protective immune response in the host, and it is a potential vaccine candidate for the prevention of Lm infection in humans and animals.