Antibacterial secondary metabolites of Clonostachys rosea, an endophytic fungus from Blumea balsamifera (L.) DC.

Xuechun Shu; Yingbo Zhang; Lingliang Guan; Zhenxia Chen; Mei Huang; Xiaolu Chen; Yuan Yuan; Chao Yuan

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Antibacterial secondary metabolites of Clonostachys rosea, an endophytic fungus from Blumea balsamifera (L.) DC.

Author: Xuechun SHU ¹ ; Yingbo ZHANG ² ; Lingliang GUAN ² ; Zhenxia CHEN ² ; Mei HUANG ² ; Xiaolu CHEN ² ; Yuan YUAN ³ ; Chao YUAN ²
Author Information

1. College of Forestry, Hainan University, Haikou 570228, Hainan, China.
2. Tropical Crops Genetic Resources Institute, CATAS/Hainan Provincial Engineering Research Center for Tropical Medicinal Plant, Danzhou 571737, Hainan, China.
3. School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong, China.
Publication Type:Journal Article
Keywords: Blumea balsamifera (L.) DC.; antimicrobial activity; endophytes; secondary metabolites
From: Chinese Journal of Biotechnology 2020;36(8):1650-1658
CountryChina
Language:Chinese
Abstract: Endophytic fungus is an important treasure trove for discovery of structurally unusual and biologically diverse compounds. A phytochemical investigation on a fungus Clonostachys rosea inhabits inner tissue of Blumea balsamifera (L.) DC. was initiatedrecently in our lab. Six pure compounds were isolated through silica gel column chromatography, sephadex LH-20, and semi-preparative HPLC techniques, with bio-guided strategy. Their structures were characterized as verticillin A (1), (S)-(+)-fusarinolic acid (2), 8-hydroxyfusaric acid (3), cerebroside C (4), 3-Maleimide-5-oxime (5), and bionectriol A (6) by analyses of NMR and MS data. All compounds were tested in vitro antibacterial activities against four strains of bacteria, Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa, and results revealed that 1, 4 and 6 display notableinhibition againstthree bacteria, with MIC values ranging from 2 to 16 μg/mL. Our findings provide references for mining novel antibiotics from endophytes originated from Li Minority medicinal plant B. balsamifera (L.) DC.