Two-in-one strategy: a remineralizing and anti-adhesive coating against demineralized enamel.
10.1038/s41368-020-00097-y
- Author:
Ailin HOU
1
;
Jun LUO
2
;
Min ZHANG
1
;
Jianshu LI
3
;
Wenlin CHU
2
;
Kunneng LIANG
1
;
Jiaojiao YANG
4
;
Jiyao LI
5
Author Information
1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
2. College of Polymer Science and Engineering, Sichuan University, Chengdu, China.
3. College of Polymer Science and Engineering & State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, China.
4. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China. jiaojiao.yang@scu.edu.cn.
5. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China. jiyaoliscu@163.com.
- Publication Type:Journal Article
- From:
International Journal of Oral Science
2020;12(1):27-27
- CountryChina
- Language:English
-
Abstract:
Tooth enamel is prone to be attacked by injurious factors, leading to a de/remineralization imbalance. To repair demineralized enamel and prevent pulp inflammation caused by biofilm accumulation, measures are needed to promote remineralization and inhibit bacterial adhesion on the tooth surface. An innovative material, poly (aspartic acid)-polyethylene glycol (PASP-PEG), was designed and synthesized to construct a mineralizing and anti-adhesive surface that could be applied to repair demineralized enamel. A cytotoxicity assay revealed the low cytotoxicity of synthesized PASP-PEG. Adsorption results demonstrated that PASP-PEG possesses a high binding affinity to the hydroxyapatite (HA)/tooth surface. In vitro experiments and scanning electron microscopy (SEM) demonstrated a strong capacity of PASP-PEG to induce in situ remineralization and direct the oriented growth of apatite nanocrystals. Energy dispersive X-ray spectroscopy (EDS), X-ray diffraction analysis (XRD) and Vickers hardness tests demonstrated that minerals induced by PASP-PEG were consistent with healthy enamel in Ca/P ratio, crystal form and surface micro-hardness. Contact angle tests and bacterial adhesion experiments demonstrated that PASP-PEG yielded a strong anti-adhesive effect. In summary, PASP-PEG could achieve dual effects for enamel repair and anti-adhesion of bacteria, thereby widening its application in enamel repair.
