Quantitative Evaluation of Sciatic Nerve Crush Injury with Conventional Ultrasound Combined with Shear-wave Elastography in Rabbit Models.
10.3881/j.issn.1000-503X.11336
- Author:
Ya-Qiong ZHU
1
;
Zhuang JIN
1
;
Si-Ming CHEN
1
;
Ling REN
1
;
Yue-Xiang WANG
1
;
Xiao-Qi TIAN
1
;
Yu-Kun LUO
1
Author Information
1. Department of Ultrasound,Chinese PLA General Hospital,Beijing 100853,China.
- Publication Type:Journal Article
- Keywords:
conventional ultrasound;
crushed injury;
sciatic nerve;
shear-wave elastography
- MeSH:
Animals;
Crush Injuries;
diagnostic imaging;
Elastic Modulus;
Elasticity Imaging Techniques;
Male;
Muscle, Skeletal;
innervation;
pathology;
Rabbits;
Random Allocation;
Sciatic Nerve;
injuries;
Ultrasonography
- From:
Acta Academiae Medicinae Sinicae
2020;42(2):190-196
- CountryChina
- Language:Chinese
-
Abstract:
To explore the value of conventional ultrasound combined with shear-wave elastography in the quantitative evaluation of sciatic nerve crush injury in rabbit models. Forty healthy male New Zealand white rabbits were randomly divided into four groups (=10 in each group):three crush injury (CI) groups (2,4,and 8 weeks after crush) and control group (without injury). The thickness and stiffness of the crushed sciatic nerves and denervated triceps surae muscles were measured at different time points and compared with histopathologic parameters. Inter-reader variability was assessed with intraclass correlation coefficients. Compared with the control group,the inner diameters of the sciatic nerves significantly increased in the 2-week CI group [(1.65±0.34) mm (0.97±0.15) mm,=0.00] but recovered to the nearly normal level in the 8-week CI group [(1.12±0.18) mm (0.97±0.15) mm,=0.06];however,compared with control group [(8.75±1.02)kPa],the elastic modulus of the nerves increased significantly in all the CI groups [2-week:(14.77±2.53) kPa;4-week:(19.12±3.46) kPa;and 8-week:(28.39±5.26) kPa;all =0.00];pathologically,massive hyperplasia of collagen fibers were found in the nerve tissues. The thickness of denervated triceps surae muscle decreased gradually,and the elastic modulus decreased 2 weeks after injury but increased gradually in the following 6 weeks;pathologically,massive hyperplasia of collagen fibers and adipocytes infiltration were visible,along with decreased muscle wet-weight ratio and muscle fiber cross-sectional area. The inter-reader agreements were good. Conventional ultrasound combined with shear-wave elastography is feasible for the quantitative evaluation of the morphological and mechanical properties of crushed nerves and denervated muscles.
