Bioinformatics Analysis and Preliminary Functional Study of Abnormal Expression of Splicing Factors in Gastric Cancer.
10.3881/j.issn.1000-503X.11414
- Author:
Aidong ZHANG
1
;
Juan SHI
1
Author Information
1. State Key Laboratory of Medical Molecular Biology,Institute of Basic Medical Sciences, CAMS and PUCM,Beijing 100005,China.
- Publication Type:Journal Article
- Keywords:
The Cancer Genome Atlas;
gastric cancer;
serine/arginine-rich splicing factor 10;
splicing factor
- MeSH:
Alternative Splicing;
Cell Cycle Proteins;
Computational Biology;
Gene Expression Regulation, Neoplastic;
Humans;
RNA Splicing Factors;
Repressor Proteins;
Serine-Arginine Splicing Factors;
Stomach Neoplasms
- From:
Acta Academiae Medicinae Sinicae
2020;42(3):289-296
- CountryChina
- Language:Chinese
-
Abstract:
To analyze the expression of splicing factors in gastric cancer using bioinformatics methods and investigate the effect of aberrantly expressed serine/arginine-rich splicing factor(SRSF10)on the phenotype of gastric cancer cells. The RNA-seq data of gastric cancer and paracancerous tissues were downloaded from The Cancer Genome Atlas(TCGA)cancer database,and bioinformatics analysis was performed to obtain the splicing factors differentially expressed in gastric cancer.The splicing factor SRSF10 was selected to investigate its effect on the development of gastric cancer.RNA interference technology was used to construct SRSF10 knockdown gastric cancer cells.MTS,Transwell,and cell scratches were used to study the effect of SRSF10 knockdown on gastric cancer cell phenotype. A total of 48 splicing factors were identified in gastric cancer by a series of bioinformatics techniques,of which 35 were up-regulated and 13 were down-regulated.The splicing factor SRSF10,which was up-regulated,was selected for further study.It was found that the gastric cancer cells after SRSF10 knockdown proliferated more slowly and had lower migration ability than normal gastric cancer cells. Multiple splicing factors are found in gastric cancer and may play an important role in the development of gastric cancer.The splicing factor SRSF10 may contribute to the pathogenesis of gastric cancer.
