Anti-inflammatory effects of IL-4 and IL-10 on Human Polymorphonuclear Leukocytes.
- Author:
Sung Woo LEE
1
;
Yun Sik HONG
;
Chung Min CHUN
;
Jun Dong MOON
;
Su Jin KIM
;
In Chul JUNG
;
Young Hoon YOON
;
Be An LEE
;
Sung Woo MOON
;
Sung Hyuk CHOI
;
Chul Kyu MOON
Author Information
- Publication Type:Original Article
- Keywords: Interleukin-4; Interleukin-10; Interleukin-8; Tumor Necrosis Factor-; N-Formylmethionine; Leucyl-Phenylalanine; Neutrophils
- MeSH: Cells, Cultured; Humans; Hydrogen Peroxide/metabolism; Interleukin-10/*pharmacology; Interleukin-4/*pharmacology; Interleukin-8/metabolism; Intracellular Fluid; N-Formylmethionine Leucyl-Phenylalanine/pharmacology; Neutrophils/cytology/*drug effects/immunology; Tumor Necrosis Factor-alpha/metabolism
- From:Journal of Korean Medical Science 2002;17(1):7-14
- CountryRepublic of Korea
- Language:English
- Abstract: Inflammatory responses are strictly regulated by coordination of pro-inflammatory and anti-inflammatory mediators. Interleukin-4 (IL-4) and interleukin-10 (IL-10) have typically the biologic anti-inflammatory effects on monocytes, but uncertain effects on polymorphonuclear leukocytes (PMNs). The PMNs are the first line of cellular response for host defense during acute inflammation. To modify hyper-inflammatory reaction with biologic anti-inflammatory mediators, we have determined the biologic anti-inflammatory activities of IL-4 and IL-10 on human PMNs. Human PMNs were pretreated with IL-4 or IL-10 and then stimulated with formyl methionyl leucyl phenylalanine (fMLP) for times indicated. The level of H2O2, interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-alpha) were determined in the each cell free supernatants. fMLP plays the role of a typical pro-inflammatory agent and, at least in determined conditions, down-regulated TNF release. IL-4 acts as an anti-inflammatory mediator but IL-10 did not show its anti-inflammatory activities on fMLP-stimulated human PMNs. IL-4 and IL-10 have different anti-inflammatory mechanisms. Perhaps, IL-10 needs co-factors to act as an anti-inflammatory mediator.
