Inhibitory effects of Capsicum annuum L. water extracts on lipoprotein lipase activity in 3T3-L1 cells.
- Author:
Jongmi BAEK
1
;
Jaesung LEE
;
Kyoungkon KIM
;
Taewoo KIM
;
Daejung KIM
;
Cheonan KIM
;
Kanazawa TSUTOMU
;
Sarangowa OCHIR
;
Kooyeon LEE
;
Cheol Ho PARK
;
Yong Jik LEE
;
Myeon CHOE
Author Information
- Publication Type:Original Article
- Keywords: Anti-obesity; Capsicum annuum L.; lipoprotein lipase (LPL); 3T3-L1 cells; RT-PCR
- MeSH: 3T3-L1 Cells; Capsaicin; Capsicum; Chloroform; Chromatography; Lipoprotein Lipase; Lipoproteins; Metabolic Diseases; Obesity; RNA, Messenger; Water
- From:Nutrition Research and Practice 2013;7(2):96-102
- CountryRepublic of Korea
- Language:English
- Abstract: Obesity, an intractable metabolic disease, currently has no medical treatment without side effects, so studies have been actively carried out to find natural compounds that have anti-obesity activity with minimum side effects. In this study, the anti-obesity effects of water extracts of seven Capsicum annuum L. varieties being Putgochu (Pca), Oyee gochu (Oca), Kwari putgochu (Kca), Green pepper (Gca), Yellow paprika (Yca), Red paprika (Rca) and Cheongyang gochu (Cca), were examined through the evaluation of lipoprotein lipase (LPL) mRNA expression level in 3T3-L1 cells (mouse pre-adipocytes). After capsaicin elimination by chloroform defatting, freeze-dried powder of Cca was treated to 3T3-L1 cells and anti-obesity effects were examined by determining the LPL mRNA level using the RT-PCR method. Of the primary fractions, only proven fractions underwent secondary and tertiary refractionating to determine anti-obesity effects. From seven different Capsicum annuum L., there was a significant decrease of the LPL mRNA expression level of 50.9% in Cca treatment compared to the control group. A significant decrease of the LPL mRNA expression level was shown in primary fractions (Fr) 5 (36.2% decrease) and 6 (30.5% decrease) of the Cca water extracts. Due to the impurities checked by UPLC chromatography, Fr 5 and 6 were refractionated to determine the LPL mRNA expression level. Treatment of Fr 6-6 (35.8% decrease) and Fr 5-6 (35.3% decrease) showed a significant decrease in the LPL mRNA expression level. When analyzed using UPLC, major compounds of Fr 6-6 and Fr 5-6 were very similar. Subsequently, we refractionated Fr 6-6 and Fr 5-6 to isolate the major peak for structure elucidation. Treatment of Fr 5-6-1 (26.6% decrease) and Fr 6-6-1 (29.7% decrease) showed a significant decrease in the LPL mRNA expression level. Consequently, the fractions may have a possibility to ameliorate obesity through the decrease of the LPL mRNA expression level.
