Study on Improvement Effects of Icariside Ⅱ on Neurological Function of Focal Cerebral Ischemia Model Rats by Regulating miR- 141-3p/Notch/Nrf2 Axis
- VernacularTitle:淫羊藿次苷Ⅱ调节miR-141-3p/Notch/Nrf2轴对局灶性脑缺血模型大鼠神经功能的改善作用研究
- Author:
Chen GAO
1
;
Yingpeng ZHI
2
Author Information
1. Dept. of Encephalopathy,Jinan Hospital of TCM,Jinan 250012,China
2. Dept. of Hyp ertension,Jinan Hospital of TCM,Jinan 250012,China
- Publication Type:Journal Article
- Keywords:
Icariside Ⅱ;
miR-141-3p;
Notch;
Nuclear factor erythroid- 2-related factor 2;
Focal cerebral ischemia model;
Rats
- From:
China Pharmacy
2020;31(19):2386-2391
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the improvement effects of icariside Ⅱ(ICS Ⅱ)on neurological function of focal cerebral ischemia model rats by regulating miR- 141-3p/Notch/nuclear factor erythroid- 2-related factor 2(Nrf2)axis(miR-141-3p/Notch/ Nrf2). METHODS :The rats were divided into sham operation group ,model group ,nimodipine group (20 mg/kg)and ICS Ⅱ low-dose,medium-dose and high-dose groups (4,8,16 mg/kg),with 20 rats in each group. Twenty-four hours after establishing focal cerebral ischemia model ,model rats were given re levant medicine or normal saline intragastrically ,twice a day ,for consecutive 3 d. The neurological deficit of rats in each group was scored ;the volume of cerebral infarction was measured by 2,3, 5-triphenyltetrazolium chloride (TTC)staining;water content of cerebral tissue and the permeability of blood-brain barrier were measured;HE staining was performed to observe the pathological change of cerebral tissue of rats ;the expression of miR- 141-3p in cerebral tissue of rats was measured by qRT-PCR ;the protein expression of Notch and Nrf 2 in cerebral tissue of rats were measured by Western blotting assay. RESULTS :Compared with sham operation group ,the neurological deficit score ,expression of Notch-1 and Nrf 2 in model group were significantly lowered (P<0.05);infarction volume ,brain water content ,the permeability of blood-brain barrier and the expression of miR- 141-3p in cerebral tissue were increased significantly (P<0.05);the distribution of cortical cells was disordered ,and inflammatory infiltration and necrosis were observed in a large number of nerve cells. Compared with model group ,the neurological deficit score ,the protein expression of Notch- 1 and Nrf 2 in cerebral tissue were significantly increased in ICS Ⅱgroups(P<0.05);infarction volume ,brain water content ,the permeability of blood-brain barrier and the expression of miR- 141-3p in cerebral tissue were decreased significantly (P<0.05);the arrangement of cortical cells was regular,and the inflammatory infiltration and necrosis of nerve cells were decreased significantly. CONCLUSIONS :ICS Ⅱ can promote the recovery of neurological function in focal cerebral ischemic model rats ,which may be related to down-regulation of miR-141-3p and activation of Notch/Nrf 2 axis.