Study on Spectrum-effect Relationship of Anti-inflammatory Effect of Different Polar Parts of Ampelopsis grossedentata in Mice Based on Grey Relational Analysis
- VernacularTitle:基于灰色关联分析的藤茶不同极性部位在小鼠体内抗炎作用的谱-效关系研究
- Author:
Wen LIU
1
;
Yun LIU
2
;
Jinbao LIU
3
;
Haijiao GUO
1
;
Lizhen ZHENG
1
;
Liyuan ZHOU
1
;
Yanni ZHONG
1
;
Jieping QIN
1
Author Information
1. College of Pharmacy，Guangxi University of TCM，Nanning 530001，China
2. Dept. of Clinical Laboratory，the Affiliated Hospital of Xuzhou Medical University，Jiangsu Xuzhou 221002，China
3. Dept. of Human Resources，International Zhuang Medical Hospital of Guangxi，Nanning 530200，China
- Publication Type:Journal Article
- Keywords:
Ampelopsis grossedentata;
Fingerprint;
Anti-inflammatory activity;
Spectrum-effect relationship;
Grey relational
- From:
China Pharmacy
2020;31(19):2382-2386
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study the spectrum-effect relationship of HPLC finger print of different polar parts of Ampelopsis grossedentata with its in vivo anti-inflammatory effect. METHODS ：A. grossedentata was reflux extracted with 70％ ethanol，then extracted with petroleum ether ，chloroform，ethyl acetate and water saturated n-butanol；or it was directly decocted with water and then concentrated to obtain different polar parts. The xylene-induced mice ear swelling model was established ；using dexamethasone as positive control ，anti-inflammatory activity of different polar parts of A. grossedentata was investigated. Fingerprints of different polar parts of A. grossedentata were established by HPLC. The determination was performed on Poroshell 120 EC-C18 column with mobile phase consisted of acetonitrile- 0.1％ phosphoric acid solution （gradient elution ）at the flow rate of 1 mL/min. The column temperature was 25 ℃. The detection wavelength was set at 365 nm，and sample size was 5 μL. The grey ralational analysis method was used to analyze the spectrum-effect relationship of HPLC fingerprint common peaks of different polar parts of A. grossedentata with its anti-inflammatory effect. The correlation coefficient and correlation degree were calculated and ranked. RESULTS：Anti-inflammatory experiment showed that the anti-inflammatory effects of 70％ ethanol extraction part ，ethyl acetate extraction part and water extraction part were the most significant （inhibitory rates of ear swelling were 54.07％，30.54％， 30.45％）. Five common peaks were determined in HPLC fingerprints of different polar parts from A. grossedentata . The spectrum-effect analysis results showed that the correlation of5 common peaks were higher than 0.6；among them ，peak 3 and peak 2 （dihydromyricetin） had the strongest anti- inflammatory effect ，and their correlation degrees were both mail：123745789@qq.com greater than 0.8. CONCLUSIONS ： The anti-inflammatory effect of A. grossedentata on xylene-induced ear swelling in mice is the result of multi-comp onent synergy ； unknown substance of peak 3 and dihydromyricetin may be the main active components of A. grossedentata .
