Study on the Effects of Deoxyschizandrin on the Proliferation ，Migration and Invasion of Human Nasopharyngeal Carcinoma Cell HONE- 1 via Met/PI 3K/Akt Signaling Pathway
- VernacularTitle:基于Met/PI3K/Akt信号通路研究五味子甲素对人鼻咽癌细胞HONE-1增殖、迁移和侵袭的影响
- Author:
Tengxiang CHEN
1
,
2
;
Li LIANG
1
,
2
;
Zhirui ZENG
1
,
2
;
Shan LEI
1
,
2
;
Jingya WANG
1
,
2
;
Yuanmei SUN
1
,
2
;
Jinzhi LAN
2
,
3
;
Yan XUE
1
Author Information
1. Dept. of Physiology，School of Basic Medical Sciences，Guizhou Medical University，Guiyang 550025，China
2. Guizhou Provincial Key Laboratory of Pathogenesis & Drug Research on Common Chronic Diseases，Guiyang 550025，China
3. Tissue Engineering and Stem Cell Research Center，Guizhou Medical University，Guiyang 550025，China
- Publication Type:Journal Article
- Keywords:
Deoxyschizandrin;
Proliferation;
Migration
- From:
China Pharmacy
2020;31(19):2376-2381
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study the effects and potential mechani sm of deoxyschizandrin on the proliferation ，migration and invasion of nasopharyngeal carcinoma cell HONE- 1. METHODS ：HONE-1 cell was set as cell model ，while CCK- 8 test，wound healing assay and Transwell chamber test were used to detect the proliferation ，migration and invasion ability changes of HONE- 1 cells after treatment with different concentrations [ 0（blank control ），10，20，40 μmol/L] of deoxyschizandrin. Computer molecular docking was performed to analyze the binding ability between deoxyschizandrin and Met protein. Western blotting assay was used to detect the relative protein expressions of p-Met ，p-PI3K，p-Akt，Bcl-2 and N-cadherin in cells. RESULTS ：Compared with blank control ，the proliferation ，migration and invasion ability of cells after treated with 10，20，40 μmol/L deoxyschizandrin were all decreased significantly （P＜0.05）. Results of molecular docking revealed that deoxyschizandrin could stably bind with the activity pocket of Met protein. Results of Western blotting assay demonstrated that compared with blank control ，10，20，40 μmol/L deoxyschizandrin all decreased the relative protein expressions of p-Met ，p-PI3K，p-Akt，Bcl-2 and N-cadherin in cells significantly（P＜0.05）. CONCLUSIONS ：Deoxyschizandrin can inhibit the proliferation ，migration and invasion of HONE- 1 cell via inhibiting the activation of Met/PI 3K/Akt signaling pathway.
