Regulatory Effects of Stilbene Glucoside on JNK and PP 2B in APP/PS1/Tau Transgenic Dementia Mice
- VernacularTitle:二苯乙烯苷对APP/PS1/Tau三转基因痴呆小鼠JNK和PP2B的调控作用研究
- Author:
Wenxue WU
1
;
Yanzhao SU
2
;
Chaoyu LIU
1
;
Junjie TAN
2
;
Zhenzhong LI
3
;
Jian HUANG
3
;
Xiaoying ZHU
4
;
Yanhua LIAO
4
;
Zhongshi HUANG
1
Author Information
1. School of Basic Medicine，Youjiang Medical University for Nationalities，Guangxi Baise 533000，China
2. School of Pharmacy，Guangxi University of TCM，Nanning 530200，China
3. School of Pharmacy，Youjiang Medical University for Nationalities，Guangxi Baise 533000，China
4. School of Clinical Medicine，Youjiang Medical Unive rsity for Nationalities，Guangxi Baise 533000，China
- Publication Type:Journal Article
- Keywords:
Alzheimer’s disease;
Stilbene glucoside;
APP/PS1/Tau transgenic dementia mice;
c-Jun N-terminal kinase
- From:
China Pharmacy
2020;31(19):2339-2345
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study the regulatory effects of stilbene glucosid e（TSG）on c-Jun N-terminal kinase （JNK）and protein phosphortase 2B（PP2B）in APP/PS1/Tau transgenic dementia （3×Tg-AD）mice，and to explore its potential mechanism of anti-Alzheimer’s disease （AD）. METHODS ：Totally 45 male 3×Tg-AD mice were randomly divided into model group ，positive control group （huperzine A ，0.15 mg/kg），TSG low-dose ，medium-dose and high-dose groups （0.033，0.1，0.3 g/kg），with 9 mice in each group. Another 9 normal male C 57BL/6J mice were included into normal control group. Administration groups were given relevant medicine intragastrically ，once a day ，for consecutive 60 d. Normal control group and model group were given constant volume of normal saline intragastrically. After medication ，Morris water maze experiment was used to test the spatial learning and memory ability of mice in each group ；Nissl staining was used to observe the changes of Nissl bodies in cerebral cortex and hippocampus ；mRNA and protein expressions of JNK and PP 2B were detected by qRT-PCR and Western blotting assay. RESULTS：Compared with normal control group ，the escape latency was significantly prolonged （P＜0.01），the retention time of the original platform quadrant was significantly shortened （P＜ and the times of crossing the platform was significantly reduced in model group （P＜0.01）；the number of Nissl bodies in cerebral cortex and hippocampus was significantly 729011126@qq.com reduced，the staining was slight ；the relative expressions of JNK mRNA and protein were significantly increased （P＜ 0.01），and the relative expressi ons of PP 2B mRNA and protein were significantly decreased （P＜0.01）. Compared with model group ，the escape latency was significantly shortened in positive control group and TSG groups （P＜0.01）；the retention time of the original platform quadrant was significantly prolonged （P＜0.01）；the times of crossing the platform was significantly increased （P＜0.01）；the number of Nissl bodies in cerebral cortex and hippocampus was increased significantly ，the staining was heavy ；the relative expression of JNK protein was significantly decreased（P＜0.05 or P＜0.01），the relative expressions of PP 2B mRNA and protein were significantly increased （P＜0.01）， while the relative expression of JNK mRNA was significantly decreased in TSG high-dose group （P＜0.05）. CONCLUSIONS ：TSG can improve the learning and memory ability and neuronal damage of 3 × Tg-AD mice. The mechanism may be related to down-regulating the transcription and expression of protein kinase JNK ，up-regulating the transcription and expression of protein phosphatase PP 2B.
