Effects of Stilbene Glucoside on the Proliferation and Estrogen Receptor Expression of Human Breast Cancer T-47D Cells
- VernacularTitle:二苯乙烯苷对人乳腺癌T-47D细胞增殖及其雌激素受体表达的影响
- Author:
Can ZHU
1
;
Yan WANG
1
;
Yaofeng LI
1
;
Yunyan FU
1
;
Wenchao TANG
1
;
Changfu YANG
1
;
Hesheng WANG
1
Author Information
1. College of Basic Medicine，Guizhou University of TCM，Guiyang 550025，China
- Publication Type:Journal Article
- Keywords:
Stilbene glucoside;
Phytoestrogen;
Human breast cancer T- 47D cells;
Cell proliferation;
Estrogen receptor
- From:
China Pharmacy
2020;31(19):2315-2320
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study the effects of stilbene glucoside （TSG）on the proliferation and estrogen receptor （ER）of human breast cancer T- 47D cells ，and to explore its estrogen-like effect and potential mechanism. METHODS ：Taking ER positive human breast cancer T- 47D cells as subjects ，using β-estradiol（β-E2，1×10－8 mol/L）as positive control ，CCK-8 assay was used to detect the cell proliferation after treated with different concentrations of TSG （1×10－8，1×10－7，1×10－6，1×10－5，1×10－4 mol/L）for 24，48，72 h；the cell proliferation rate was calculated. Western blotting assay and RT-PCR methods were adopted to detect the protein and mRNA expression of ER-α and ER-β in cells after treated with low，medium and high concentrations of TSG （1×10－8， 1×10－6，1×10－4 mol/L）for 48 h. RESULTS ：After treated with different concentrations of TSG for 24，48，72 h，the cell proliferation rate of each administration group at each time point （except for β-E2 group at 48 h）increased significantly ，compared with blank group ；those of TSG groups （1×10－5，1×10－6，1×10－7 mol/L）were significantly higher than β-E2 group（P＜0.05 or P＜0.01）. After treated with low ，medium and high concentrations of TSG for 48 h，protein and mRNA expression of ER-α and ER-β in cells were increased significantly，compared with blank group （P＜0.05 or P＜0.01）；protein expression of ER-β in TSG low concentration group ，mRNA expression of ER-α in TSG groups as well as mRNA expression of ER-β in TSG low and high concentration groups were significantly higher than β-E2 group（P＜0.05 or P＜0.01）. CONCLUSIONS ：TSG can induce the in vitro proliferation of T- 47D cells and exert estrogen-like effects by promoting protein and mRNA expression of ER-α and ER-β， which is stronger than that of β-E2 at a certain concentration.
