Study on pigmentation in the livers and kidneys of rats caused by a compound Chinese medicine preparationXIA
10.19485/j.cnki.issn2096-5087.2020.10.008
- VernacularTitle:某复方中药制剂引起大鼠肝肾色素沉积的研究
- Author:
Xia Yong
1
;
XU Caiju
;
LU Wei
;
SONG Yanhua
;
DONG Xiaolan
;
FU Jianyun
;
ZHENG Xiaoyan
Author Information
1. Zhejiang Provincial Center for Disease Control and Prevention
- Publication Type:Journal Article
- Keywords:
traditional Chinese medicine preparation gardenia pigmentation bile pigment
- From:
Journal of Preventive Medicine
2020;32(10):1004-1009
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the cause and type of pigmentation in the livers and kidneys of rats caused by a compound Chinese medicine preparation.
Methods:The experiment consist of low, medium, and high dose groups and a control group, the Sprague-Dawley rats in these groups were orally given 1.0, 2.0 and 4.0 g/kgbw of a compound Chinese medicine preparation of gardenia and distilled water for 30 days, respectively. The body weight, diet, hematology and histopathology of the rats in each group were observed for changes in pigment metabolism.
Results:In the first and second weeks of the experiment, the rats in the low, medium, high dose groups and the control group showed no abnormal symptoms or signs. From the third weekend, the urine of the rats in the high dose group turned thick yellow and green, and the stool color became light. During the experiment, no rats died. There were statistically significant differences in body weights and weight gains among these groups ( P<0.05 ) . There were statistically significant differences in total food utilization, direct bilirubin, r-glutamyl transferase, alkaline phosphatase, and liver/body ratios among these groups ( P<0.05 ). Gross examination revealed that the livers and kidneys of rats in the high dose group were dull and green. Microscopic examination revealed changes in dark pigment particles in the livers and kidneys of rats in the high dose group. Histochemical staining confirmed that pigments in the livers and kidneys were bile pigments.
Conclusions:At a dosage of 4.0 g/kgbw, a compound Chinese medicine preparation of gardenia can lead to bile pigment deposition in the livers and kidneys of rats due to cholestasis.
- Full text:某复方中药制剂引起大鼠肝肾色素沉积的研究.pdf
