Effect of allogeneic platelet transfusion on migration and invasion of human lung cancer A549 cells and its mechanism of action
10.3872/j.issn.1007-385x.2020.09.009
- VernacularTitle:异体血小板输注对人肺癌A549细胞迁移和侵袭的影响及其作用机制
- Author:
HAN Lina
1
,
2
;
ZHAO Xuetao
1
,
2
;
MA Ming
1
,
2
;
WU Bo
1
,
2
;
ZHAO Liang
1
,
2
;
ZHANG Cong
1
,
2
;
SHAN Baoen
1
,
2
Author Information
1. (a. Department of Blood Transfusion
2. b. Department of Laboratory c. Scientific Research Center, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China
- Publication Type:Journal Article
- Keywords:
blood platelet;
lung cancer;
A549 cell;
migrations;
invasion;
epithelial-mesenchymal transition (EMT);
vascular endothelial growth factor (VEGF);
Matrix metalloproteinase(MMPs)
- From:
Chinese Journal of Cancer Biotherapy
2020;27(9):1018-1023
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To observe the effect of allogeneic platelets transfusion on the invasion and metastasis of human lung cancer A549 cells, and to preliminarily explore its mechanism of action. Methods: Eighty-nine patients with advanced lung cancer, who had received platelet transfusion in the Chemotherapy Department of Fourth Hospital of Hebei Medical University between January 2017
and December 2018, were enrolled in this study. The study cells were randomized into Ctrl group (A549 cells co-incubated with culture medium), Before group, and After group (A549 cells co-incubated with plasma Before and After platelet transfusion, respectively). The migration and invasion of A549 cells co-cultured with plasma before and after platelet transfection were detected by Scratch and
Transwell experiments. The expression of MMPs, TIMPs and epithelial-mesenchymal transition (EMT) related proteins E-cadherin, N-cadherin and Vimentin, as well as vascular endothelial growth factor (VEGF) and its receptor 2 (VEGFR2) were detected by Western blotting (WB) method. Results: The scratch healing ability of A549 cells in After group was significantly higher than that of Ctrl group
and Before group [(73.67±2.60)% vs (58.33±2.33)%, (35.33±2.03) %; P<0.01, vs Ctrl group; P<0.05, vs Before group], and there was also a significant difference between Before group and Ctrl group (P<0.05). The results of cell migration experiment showed that the number of transmembrane cells in After group was significantly higher than that in Ctrl group and Before group [(69.67±7.84) vs (18±2.08) and (39.33±2.03), all P<0.01]. The cell invasion experiment showed that the number of transmembrane cells in After group was significantly higher than that in Ctrl group and Before group [(59.34±3.46) vs (18.34±1.56) and (37.58±2.79), all P<0.01]. When A549 cells were co-incubated with plasma before and after platelet transfusion for 48 h, it was found that the expressions of MMP9 and MMP2 were increased (P<0.05), while their inhibitors TIMP1 and TIMP2 were decreased (P<0.01); the expressions of EMT-related proteins N-cadherin and Vimentin were increased (P<0.05), but E-cadherin was decreased (P<0.01); the expressions of angiogenesis related proteins VEGF and VEGFR2 were increased (P<0.05). Conclusion: Alloplatelets transfusion can promote the invasion and metastasis of lung cancer A549 cells, which may be realized by regulation of the expressions of EMT, metallomatrix protease and vascular growth factor-related proteins.
- Full text:20200909.pdf
