Preliminary screening and analysis of differentially expressed circRNAs in esophageal squamous cell carcinoma
10.3872/j.issn.1007-385x.2020.09.008
- VernacularTitle:食管鳞状细胞癌差异性表达circRNA的初步筛选和分析
- Author:
ZHANG Dehai
1
;
FU Zheng
2
,
3
;
WANG Jian
4
;
WANG Guojun
4
;
HUANG Junxian
2
,
3
;
CHEN Xi
2
,
3
Author Information
1. 1. Department of Thoracic Surgery, Jintan Hospital Affiliated to Jiangsu University, Jintan 213200, Jiangsu, China
2.
3. 2. College of Life Sciences, Nanjing University, Nanjing 210023, China
4. （1. Department of Thoracic Surgery, Jintan Hospital Affiliated to Jiangsu University, Jintan 213200, Jiangsu, China
- Publication Type:Journal Article
- Keywords:
esophageal squamous cell carcinoma;
circRNA;
differential expression;
biological function;
high throughput sequencing
- From:
Chinese Journal of Cancer Biotherapy
2020;27(9):1012-1017
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To construct a circRNA profile of esophageal squamous cell carcinoma (ESCC) and analyze differentially expressed circRNAs. Methods: Samples were taken from 3 patients with esophageal squamous cell carcinoma who were hospitalized in the Department of Thoracic Surgery, Jintan Hospital, Jiangsu University from June 2018 to February 2019. The circRNA expression
profile was constructed by high-throughput sequencing technique, and the circRNA differentially expressed in 3 pairs of esophageal squamous cell carcinoma tissues and adjacent tissues was detected. The biological functions and related signal pathways of these circRNA were analyzed by GO and KEGG techniques. Results: By comparing the expression levels of circRNA between esophageal
squamous cell carcinoma and adjacent tissues, 905 differentially expressed circRNA were found, of which 404 were up-regulated and 501 were down-regulated. hsa_circ_0004390 was the CIRC RNA with the highest up-regulation factor (FC=7.9712), and novel_circ_0012687 was the one with the highest down-regulation factor. GO and KEGG analysis showed that these circRNA may be involved in
biological processes such as cell cycle, cell components and protein binding of cancer cells, and signal pathways such as Hippo and cGMP-PKG. Conclusion: The expression profile analysis of circRNA in esophageal squamous cell carcinoma showed that the significantly differentially expressed circRNA could be used as a potential biomarker of esophageal squamous cell carcinoma.
- Full text:20200908.pdf
