Role of lncRNA SNHG5 in the migration and invasion of hypoxia-induced hyhepa‐ tocellular carcinoma cells
10.3872/j.issn.1007-385x.2020.09.003
- VernacularTitle:lncRNA SNHG5在缺氧诱导肝细胞癌细胞侵袭及迁移中的作用
- Author:
LI Yarui
1
,
2
,
3
;
GUO Dan
1
,
2
,
3
;
CHEN Yifei
1
,
2
,
3
;
WANG Ruhua
1
,
2
,
3
;
LU Guifang
1
,
2
,
3
;
REN Mudan
1
,
2
,
3
;
LU Xinlan
1
,
2
,
3
;
HE Shuixiang
1
,
2
,
3
Author Information
1. Department of Gastroenterology, the First Affiliated Hospital of Xi'
2. an Jiaotong University, Xi'
3. an 710061, Shaanxi, China
- Publication Type:Journal Article
- Keywords:
long non-coding RNA (lncRNA);
SNHG5;
hepatocellular carcinoma (HCC);
HepG2 cell;
MHCC-97L cell;
hopoxia;
invasion;
migration
- From:
Chinese Journal of Cancer Biotherapy
2020;27(9):978-983
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To explore the regulatory effect of long non-coding RNA (lncRNA) SNHG5 on invasion and migration of
hypoxia-induced hepatocellular carcinoma (HCC) cells. Methods: A total of 20 pairs of cancer and para-cancerous tissue specimens
resected from HCC patients in the First Affiliated Hospital of Xi'an Jiaotong University from January 2017 to June 2018, and human
HCC cell lines (HepG2, MHCC-97L, MHCC-97H , Huh7) as well as immortalized human liver LO2 cells were collected for this study.
Bioinformatics methods were used to analyze the binding sites between hypoxia-inducible factor 1α (HIF-1α) and SNHG5. pCMVHIF-1α and shRNA-SNHG5 (sh-SNHG5) plasmids were transfected into HCC cells, respectively. qPCR was used to detect the expres‐
sion level of SNHG5 in HCC tissues and hypoxia-induced HCC cells. Western botting was used to detect the expression level of HIF-1α
protein in HCC cells, and Transwell chamber method was used to detect the migration and invasion ability of HCC cells after SNHG5 si‐
lence under normoxia and hypoxia condition. Results: Compared with para-cancerous tissues and immortalized human liver LO2 cells,
the expression of SNHG5 was significantly up-regulated in HCC tissues and cell lines (all P<0.01). Hypoxia promoted the expression
level of SNHG5 in HCC cells, and its mechanism might be related to the combination of hypoxia-activated HIF-1α and SNHG5
promoter to promote its transcription. Hypoxia promoted the invasion and migration ability of HepG2 and MHCC-97L cells (all P<
0.01), but knockdown of SNHG5 significantly inhibited the invasion and migration ability of HepG2 and MHCC-97L cells under hy‐
poxic conditions (all P<0.01). Conclusion: SNHG5 is highly expressed in HCC tissues and cell lines and plays an important role in the
invasion and migration of HCC cells induced by hypoxia.
- Full text:20200903.pdf
