Drug resistance characteristics and epidemic trend of Escherichia coli in Anning, Yunnan
10.3969/j.issn.1006-2483.2020.04.014
- VernacularTitle:云南安宁地区大肠埃希氏菌的耐药特征及流行趋势
- Author:
Cunfang LIU
1
;
Yan WANG
1
;
Rong YANG
1
;
Lixiang HU
1
Author Information
1. Department of Laboratory, Yunnan Kungang Hospital, Anning , Yunnan 650300, China
- Publication Type:Journal Article
- Keywords:
Escherichia coli;
Extended-spectrum β-lactamase;
Integron;
Drug resistance
- From:
Journal of Public Health and Preventive Medicine
2020;31(4):58-61
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the drug resistance characteristics and epidemic trend of Escherichia coli in Anning, Yunnan, and to provide a reference for clinical rational use of antibiotics. Methods A total of 376 strains of Escherichia coli were isolated from June 2018 to June 2019 from Kungang Hospital of Yunnan Province. The ESBLs producing strains were screened by double disk method and the sensitivity of ESBLs to antibiotics was detected by K-B method. The genotyping of ESBLs strains and the class I, II and III integrase genes in integron 3' and 5' conservative regions was conducted using polymerase chain reaction (PCR). Results The detection rate of ESBLs producing Escherichia coli was 53.19% (200/376). ESBLs-producing Escherichia coli had a low resistance rate to carbapenem antibacterial drugs with a relatively high sensitivity. The resistance rates to amikacin and piperacillin were 7.50% and 12.50% respectively, which had good antibacterial activity. The resistance rate to other antibiotics was high. There were 74.00% (148/ 200) of ESBLs-producing Escherichia coli carrying CTX-M gene, 34.50% (69/200) carrying TEM genes, and 1.00% (2/200) of strains carrying SHV genes. In addition, there were 9.00% (18/200) of strains carrying both CTX-M and TEM genes, and 0.50% (1/200) of strains carrying both CTX-M and SHV genes. The detection rate of integrons accounted for 37.00% (74/200), all of which were class I integrons. Conclusion The prevalence of ESBLs-producing Escherichia coli was relatively high in Anning, Yunnan. ESBLs-producing Escherichia coli showed a trend of multi-drug resistance. The genotype was mainly CTX-M, and the integron was classified as class I.
