Establishment of HPLC Fingerprint of Schisandra sphenanthera and S. chinensis and Analysis of Chemical Pat- tern Recognition
- VernacularTitle:南、北五味子药材的HPLC指纹图谱建立及化学模式识别分析
- Author:
Li GUO
1
;
Zhongjie YANG
1
;
Xiaotao YU
1
;
Lu JIA
2
;
Shaoju JIN
3
;
Rui WANG
1
Author Information
1. Dept. of Pharmacy，Luohe Municipal Central Hospital/Henan Engineering Research Center，Henan Luohe 462005，China
2. School of Pharmaceutical Sciences，Zhengzhou University，Zhengzhou 450002，China
3. Dept. of Pharmacology，Luohe Medical College/Tumor Occurrence and Prevention Research Innovation Team of Luohe，Henan Luohe 462002，China
- Publication Type:Journal Article
- Keywords:
Schisandra sphenanthera;
Schisandra chinensis;
HPLC;
Fingerprint;
HCA;
PCA;
OPLS-DA;
Differential
- From:
China Pharmacy
2020;31(18):2224-2229
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To establish HPLC fingerprint of Schisandra sph enanthera and S. chinensis，and to analyze chemical pattern recognition. METHODS ：HPLC method was adopted. Using schizandrin A as reference ，HPLC fingerprints of 10 batches of S. sphenanthera and S. chinensis （N1-N10，S1-S10） were drawn. Similarity Evaluation System of TCM Chromatographic Fingerprint（2012 edition）was adopted for similarity evaluation to determine the common peaks. SPSS 20.0 and SIMCA 14.1 software were used for HCA ，unsupervised madel of PCA ，supervised model of OPLS-DA. Using variable importance projection （VIP）value greater than 1 as the standard ，the differential markers that affected the quality of S. sphenanthera and S. chinensis were screened. RESULTS ：S. sphenanthera and S. chinensis were identified 32 and 33 common peaks ，respectively. The similarity of 10 batches of S. sphenanthera and 10 batches of S. chinensis were all higher than 0.9，and the similarity of S. sphenanthera and S. chinensis was 0.05. A total of 19 characteristics peaks were identified ，among which five common peaks were identified as schisandraol A ，schisandraol B ，schisantherin A ，schizandrin A and schisandrin B by reference. HCA results showed that N 1-N10 were clustered into one category ，and S 1-S10 were clustered into one category ，of which N 1，N3，N8，and N 9 were clustered into one category ，and the rest were clustered into one category ；S1，S3，S6，and S 9 were grouped together ，and the rest were grouped together. The results unsupervised model of PCA showed that the cumulative variance contribution rate of the first two principal component factors was 87.20％. Supervised model of OPLS-DA showed that schizandrin A ，schisandraol A ，schisantherin A and schisandrin B were the differential markers that affected 、the quality of S. sphenanthera and S. chinensis （VIPs were 2.29，2.24，1.73，1.48，respectively）. CONCLUSIONS ：The established fingerprint is accurate ，scientific，simple and easy to use ，combined with multivariate statistical analysis can be 话：0395-3356116。E-mail：wangrui56116@163.com used to evaluate the quality of S. sphenantherae and S. chinensis. The components of S. sphenanthera and S. chinensis were different ，schisanolrin A is differential marker.
