Study on the Spectrum-effect Relationship of Different Solvent Extracts from Trollius chinensis against Hypoxia/ reoxygenation Injury of Cardiomyocyte
- VernacularTitle:金莲花不同溶剂提取物抗心肌细胞缺氧/复氧损伤的谱-效关系研究
- Author:
Minlun NAN
1
;
Qi SUN
2
;
Zhen YANG
1
;
Xueling SI
3
;
Chunxia MA
4
;
Xue BAI
4
;
Yufang HE
5
Author Information
1. Institute of Phytochemistry，Jilin Academy of Chinese Medicine Sciences，Changchun 130012，China
2. Jilin Zhengrong Medical Development Co.，Ltd.，Jilin Dunhua 133700，China
3. Xiuzheng Pharmaceutical Group Changchun High-tech Pharmaceutical Co.，Ltd.，Changchun 130012，China
4. College of TCM，Jilin Agricultural University，Changchun 130118，China
5. College of Health Management，Changchun University of TCM，Changchun 130117，China
- Publication Type:Journal Article
- Keywords:
Trollius chinensis;
Myocardial cells H 9c2;
Hypoxia/reoxygenation injury;
Spectrum-effect relationship;
GRA;
PLS
- From:
China Pharmacy
2020;31(18):2219-2223
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study spectrum-effect relationship of 11 different solvent extracts from Trollius chinensis against hypoxia/reoxygenation injury of cardiomyocyte . METHODS ：HPLC-MS/MS method was used to establish the fingerprints of 11 different solvent extracts from T. chinensis ，the compounds corresponding to the common peaks were identified by comparing with the substance control and literature information. MTT assay was used to detect the effects of 11 different solvent extracts from T. chinensis on the survival rate of rat myocardial H 9c2 cells injured by hypoxia/reoxygenation. The MDA content ，ROS level in cells and LDH content in the supernatant were detected by ELISA. GRA and PLS method were used to analyze the spectrum-effect relationship between the compounds corresponding to common peak and anti-hypoxia/reoxygenation injury of cardiomyocyte （drug effect）. RESULTS ：There were 22 common peaks in 11 different solvent extracts from T. chinensis ，and 22 compounds were identified. Compared with hypoxia/reoxygenation injury group ，survival rate of hypoxia/reoxygenation injury+S 1-S6，S9 and S 10 groups were increased significantly ，while MDA content ，ROS level and LDH content were decreased significantly （P＜0.05）； ROS level and LDH content of hypoxia/reoxygenation injury+S 8 group w ere decreased significantly （P＜0.05）. The r of GRA analysis of 22 compounds with drug effects were all higher than 0.8. Except for peaks 1，2，7，13，14 and 21，r of PLS analysis of rest peaks with drug effects were higher than 0 发。电话：0431-86058683。E-mial：nml2000@163.com （being positive correlation ）. Top 9 common peaks in the list of contribution rate were peak 6＞11＞4＞5＞8＞9＞12＞10＞15. CONCLUSIONS ：Orientin（peak 6），vitexin（peak 11）， orientin-2″-O-β-L-galacto- pyranosl （peak 4），orientin-2″-O-β-D-Pyrine xylosides （peak 5），quercetin-3-O-glucopyranoside（peak 8），vitexin-2″-O-β-L-galactoside（peak 9），hyperoside（peak 12），vitexin-2″-O-β-D-pyrine xylosides （peak 10），2″-O-（2″′- methylbutyry-loxy）-orientin（peak 15）may be the main components of anti-hypoxia/reoxygenation injury of cardiomyocytes.
