Reduced susceptibility of Burkholderia pseudomallei following exposure to carbapenem
https://doi.org/10.47665/tb.37.3.783
- Author:
Zamani, A.
1
;
Zueter, A.R.
2
,
3
;
Muhd Besari, A.
4
,
5
;
Hasan, H.
2
,
5
;
Harun, A.
2
,
5
;
Deris, Z.Z.
2
,
5
Author Information
1. Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia
2. Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia &
3. Faculty of Allied Health Sciences, the Hashemite University, Zarqa, Jordan
4. Infectious Diseases Unit, Department of Medicine, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia &
5. Hospital Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
- Publication Type:Journal Article
- From:Tropical Biomedicine
2020;37(No.3):783-790
- CountryMalaysia
- Language:English
-
Abstract:
Reduced susceptibility in Burkholderia pseudomallei during carbapenem therapy may lead to treatment failure. We isolated a clinical strain that had developed reduced susceptibility to carbapenems while on treatment. After reviewing the patient’s clinical notes, the initial isolate (BUPS01/14) was exposed to carbapenem in vitro to mimic the clinical scenario. The stability of susceptibility of the carbapenem-exposed strain (BUPS01/14R) was examined by serial subculture in antibiotic-free broth. Biochemical and morphological comparison was performed by the VITEK® system and electron microscopy. MICs increased 32-fold following carbapenem exposure and became stable in the antibiotic-free environment. On electron microscopic examination, the BUPS01/14R cells were smoother and less wrinkled compared to BUPS01/14 cells. This report highlights a potential anti-melioidosis treatment failure due to the emergence of resistance while on carbapenem monotherapy. Further study of this strain is necessary to understand the mechanism of resistance at a molecular level.
- Full text:8.2020my1164.pdf
