Investigation of the extraction method and content determination of the active components from oral ulcer film
10.12206/j.issn.1006-0111.202003033
- VernacularTitle:口腔溃疡膜有效成分提取方法的考察及含量测定
- Author:
Shun CHEN
1
;
Lili CUI
2
,
3
;
Shouhong GAO
1
;
Wenquan LU
1
;
Jiangli SONG
1
;
Zhipeng WANG
1
Author Information
1. Department of Pharmacy, Changzheng Hospital Affiliated to Naval Medical University, Shanghai 200003, China.
2. Department of Pharmacy, Changzheng Hospital Affiliated to Naval Medical University, Shanghai 200003, China
3. School of Chemical and Biological Engineering, Yichun University, Yichun 336000, China.
- Keywords:
oral ulcer film;
dexamethasone sodium phosphate;
clotrimazole;
content determination;
HPLC
- From:
Journal of Pharmaceutical Practice
2020;38(5):466-468
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the extraction methods for active components from oral ulcer film and optimize the determination methods of active components dexamethasone sodium phosphate and metronazole. Methods Different extraction solvents(methanol, water and 70% methanol aqueous) were applied to extract the active components dexamethasone sodium phosphate and metronazole from oral ulcer film, which contents were quantified by a HPLC method. Results the extraction solvent water had the best efficacy and more simpler compared to the other two solvents. Clotriazole showed a good linear relationship within 5.014 5-200.5800 μg/ml (r=0.999 8), and the average extraction recovery was (104.23±0.63)%, and for dexamethasone sodium phosphate, a good linear relationship was obtained in the range of 0.482-16.328 μg/ml (r=0.9999), and the average extraction recovery was (103.97±1.02)%. Conclusion The water extraction method established in this study was simple and efficient, which showed features of simplicity, accuracy and repeatable.
