Simultaneous determination of five active components in Fangshu Qingre mixture by RP-HPLC
10.12206/j.issn.1006-0111.202002059
- VernacularTitle:RP-HPLC法同时测定防暑清热饮中5种活性成分的含量
- Author:
Jie LI
1
;
Yuru YANG
1
;
Qingfen WANG
1
;
Jinshan CHEN
1
Author Information
1. Department of Pharmacy, No. 909 Hospital of Joint Logistic Support Force/Southeast Hospital Affiliated to Xiamen University, Zhangzhou 363000, China.
- Keywords:
Fangshu Qingre mixture;
chlorogenic acid;
luteolin-7-O-β-D-glucoside;
3,5-dicaffeoylquinic acid;
linarin;
pogostone;
HPLC
- From:
Journal of Pharmaceutical Practice
2020;38(5):462-465
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for simultaneous determination of chlorogenic acid, luteolin-7-O-β-D-glucoside,3,5-dicaffeoylquinic acid, linarin and pogostone in Fangshu Qingre mixture by RP-HPLC. Methods ZORBAX-SB-C18 column(4.6 mm×250 mm, 5 μm) was used as the chromatographic column.The mobile phase was 0.2% formic acid water solution(A) and acetonitrile solution (B)with a gradient elution mode. The flow rate was 1.0 ml/min.The detective wavelength was 327 nm. The column temperature was 30 ℃. Results There were good linear relationships in the determination of chlorogenic acid, luteolin-7-O-β-D-glucoside, 3,5-dicaffeoylquinic acid, linarin and pogostone (r≥0.999 6), with the average recovery rate of 102.03%(1.63%), 102.38%(1.51%), 102.39%(1.23%), 103.14%(1.87%) and 104.01%(2.33%). Conclusion The method was simple and stable with a good reproducibility, which could be used as a quality control method for active compotents in Fangshu Qingre mixture.
