Identification of chemical components and monosaccharide assay in Nocardia rubra cell wall skeleton
10.12206/j.issn.1006-0111.202003073
- VernacularTitle:红色诺卡菌细胞壁骨架中化学成分的鉴别与单糖含量测定
- Author:
Zhong WAN
1
;
Dan YU
2
;
Fei WANG
2
;
Wei LI
2
;
Hai ZHANG
3
Author Information
1. Department of Urology, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai 200080，China.
2. School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355，China.
3. Department of Pharmacy, Shanghai First Maternity and Infant Hospital, Tongji University, Shanghai 201204，China.
- Keywords:
Nocardia rubra cell wall skeleton;
chemical component;
identification;
monosaccharide;
determination
- From:
Journal of Pharmaceutical Practice
2020;38(5):423-430
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze and identify the chemical components in the Nocardia rubra cell wall skeleton (Nr-CWS), and to determine the contents of monosaccharides accurately. Methods The extract of Nr-CWS was separated and analyzed by UHPLC-Q-TOF/MS method. The chemical components were quickly identified by matching the data with the information in the Metlin database. The monosaccharide contents in the Nr-CWS extract were determined by UHPLC-MS/MS method after derivatization. Results A total of 64 chemical components were identified in the extract of Nr-CWS, including amino acids, monosaccharides and so on. A assay method for 8 monosaccharides by UHPLC-MS/MS was successfully established. The content of arabinose in Nr-CWS was the highest, followed by galactose, which indicated that the main polysaccharide components in Nr-CWS may be composed of these monosaccharides. Conclusion In this study, we analyzed the main chemical components of Nr-CWS, which are amino acids, fatty acids and so on. The content of monosaccharide after polysaccharide hydrolysis was determined by UHPLC-MS/MS. This will lay a foundation for the screening of the active components of Nr-CWS and the study of its pharmacological mechanism.
