Study on secondary metabolites from sponge-symbiotic Streptomyces sp. LHW2432
10.12206/j.issn.1006-0111.202001071
- VernacularTitle:海绵共附生菌Streptomyces sp. LHW2432的次级代谢产物研究
- Author:
Liu ZHANG
1
;
Yaoyao SHEN
1
;
Lili HONG
1
;
Lei LI
1
;
Yongjun ZHOU
1
;
Houwen LIN
1
Author Information
1. Department of Pharmacy, Renji Hospital Affiliated to School of Medicine, Shanghai Jiaotong University, Shanghai 200127, China.
- Keywords:
sponge-symbiotic bacterium;
streptomyces;
secondary metabolites;
antimicrobial activity
- From:
Journal of Pharmaceutical Practice
2020;38(5):418-422
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discover the medicinal active molecules from the fermentation extract of sponge-symbiotic Streptomyces sp. LHW2432. Methods Compounds were isolated and purified from the fermentation extract of LHW2432 by silica gel, ODS chromatographic columns, and HPLC. The structures of the compounds were elucidated based on the analyses of modern spectrum technologies and the related literatures research. Through plate coating method and broth microdilution method, the antimicrobial activities were tested by the indicator strains of Bacillus mycoides, methicillin-resistant Staphylococcus aureus (MRSA), Mycobacterium smegmatis, Candida Albicans, and Escherichia coli. Results Five compounds were discovered and their structures were identified as descycloavandulyl-lavanduquinocin (1), N-acetyltyramine (2), phomapyrone C (3), germicidin A (4), and germicidin I (5). Compound 1 showed inhibitory activities against MRSA (MIC, 100 μg/ml) and M. smegmatis (MIC, 64 μg/ml), respectively. Conclusion Five compounds were discovered from LHW2432, among which compound 1 was a new natural product and could be used as a precursor of the tricyclic carbazole alkaloids with neuroprotective activity. Moreover, compound 1 showed weak inhibitory activities against gram-positive pathogenic bacteria.
