Dynamic changes of lymphocyte subsets and their correlation with renal function in recipients with stable graft status after renal transplantation
10.3969/j.issn.1674-7445.2020.05.005
- VernacularTitle:肾移植术后稳定状态受者淋巴细胞亚群的动态变化及其与肾功能的相关性分析
- Author:
Xihui MA
1
;
Yong HAN
;
Binyu LI
;
Xiangrui KONG
;
Yujie SUN
;
Li XIAO
Author Information
1. Organ Transplantation Institute of the 8th Medical Center of Chinese PLA General Hospital, Beijing Key Laboratory of Organ Transplantation and Immunology Regulatory, Beijing 100091, China
- Publication Type:Research Article
- Keywords:
Renal transplantation;
Transplantation immunity;
Lymphocyte subset;
Flow cytometry;
T cell;
Natural killer cell;
B cell;
Serum creatinine;
Blood urea nitrogen
- From:
Organ Transplantation
2020;11(5):559-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the dynamic changes of peripheral blood lymphocyte subsets and their correlation with renal function in recipients with stable graft status after renal transplantation. Methods Forty-five recipients who underwent renal transplantation for the first time and had stable graft function within postoperative 6 months were selected. The proportion and absolute value of lymphocyte subsets were detected by flow cytometry (FCM) in 180 peripheral blood samples from recipients at 15 d, 1, 3 and 6 months after renal transplantation. The dynamic changes of lymphocyte subsets with the extension of postoperative time and their correlation with serum creatinine (Scr) and blood urea nitrogen (BUN) were analyzed. Results The Scr levels did not significantly differ at 4 time points after renal transplantation (all P > 0.05). The BUN levels significantly differed between 15 d and 1 month after renal transplantation, and between 1 and 3 months after renal transplantation (P=0.002, P=0.001). The proportion of CD3+CD8+T cells, CD3+CD4+T cells, natural killer (NK) cells and CD4/CD8 ratio at postoperative 15 d significantly differed from those at 1 month after operation (P=0.009, P=0.004, P < 0.001, P=0.004). The proportion of B cells significantly differed between 15 d and 1 month, and between 1 and 3 months after renal transplantation (both P < 0.001). The absolute values of CD3+T cells, CD3+CD8+T cells, CD3+CD4+T cells and NK cells at postoperative 15 d significantly differed from those at 1 month after renal transplantation (P=0.001, P=0.002, P=0.003, P < 0.001). The absolute values of CD3+CD8+T cells significantly differed between 3 and 6 months after operation (P=0.015). The absolute value of B cells at 1 month after renal transplantation significantly differed from that at 3 months after renal transplantation (P=0.001). The proportion and absolute value of lymphocyte subsets were not significantly correlated with the Scr level (both P > 0.05). The proportion and absolute value of CD3+CD8+T cells and NK cells were negatively correlated with BUN (P < 0.001-0.05), whereas the proportion of CD3+CD4+T cells and B cells was positively correlated with the BUN level (P < 0.001-0.05). The absolute value of CD3+T cells was negatively associated with the BUN level (P < 0.05). Conclusions T cells and NK cells in the lymphocyte subsets of stable recipients raise to the stable state within 1 month after renal transplantation, whereas B cells decrease to stable state within 3 months renal transplantation. The dynamic changes of lymphocyte subsets are correlated with the BUN level.
