The Effect of Tocotrienol-Rich Fraction Supplementation on the Ovarian Metabolome and the Quality of Oocyte in Aging Mice
- Author:
NORRABIÁTUL ADAWIYAH A
;
TEH LK
;
FATHIMAH M
;
NURALIZA AS
- Publication Type:Journal Article
- Keywords:
aging, metabolomics;
ovary;
oocytes;
tocotrienol
- From:Medicine and Health
2020;15(1):54-69
- CountryMalaysia
- Language:English
-
Abstract:
Ovarian aging has been associated with oxidative stress and loss of ovarian
function. Tocotrienol has been proven to exert beneficial effects on the female
reproductive system. However, the role of tocotrienol in affecting metabolism
in the ovary and subsequently improving the quality of oocytes in aging mice
remains unknown. Therefore, the relationship between metabolic changes in the
ovary and the quality of oocytes in aging mice following tocotrienol-rich fraction
(TRF) supplementation was investigated. Six-week-old female mice were used as
the Young group. Six-month-old aged female mice were divided into four groups;
the first group was given tocopherol-stripped corn oil (vehicle control) while the
other three groups were supplemented with TRF at the dose of 90, 120, and 150
mg/kg, respectively. The treatment was given orally for two months. At the end
of the treatment, mice from all groups were superovulated and then euthanised.
Oocyte quality was assessed and non-targeted metabolomic analysis of the ovarian
tissues was performed using liquid chromatography-tandem mass spectrometry of
quadrupole time-of-flight (LC-MS Q-TOF). Percentages of normal oocytes were
higher (p<0.001) while abnormal oocytes were lower (p<0.001) in TRF 150 mg/kg
group compared to that of the control. Seventeen metabolites were identified to
be significantly different in the ovarian tissue of the aging group when compared
to the young group. 14 metabolites were identified to be significantly different in
the ovarian tissue between the control and TRF supplemented groups. Pathway
analysis revealed significantly altered metabolic pathways for fatty acid and
amino acid metabolism that might influence the quality of oocytes. In conclusion,
TRF supplementation causes metabolic changes in the ovary that delay the
consequences of aging, thus improving the quality of oocytes in aging mice.
- Full text:22.2020my0034.pdf
