The Role of ISL1 and LHX5 LIM Homeobox Genes in Bladder Tumourigenesis
- Author:
Mohd Khairul Anuar Md Akhir
;
Chan Soon Choy
;
Maizaton Atmadini Abdullah
;
Fauzah Abd Ghan
;
Abhi Veerakumarasiv
;
Huzlinda Hussin
- Publication Type:Journal Article
- Keywords:
bladder cancer;
LIM homeobox;
tumourigenesis
- From:Malaysian Journal of Medical Sciences
2020;27(1):37-45
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Lin-11, Isl-1 and Mec-3 domains (LIM) homeobox genes are among the most
important sub-families of homeobox genes. These genes are thought to play an important role in
cancer. In this study, the protein expression of these genes was examined in urothelial carcinoma
of the bladder. The expression pattern of Islet-1 (ISL1) and LIM homeobox 5 (LHX5) across
different cancer stages and grades, as well as the association between the protein expression of
these genes and patient demographics and clinicopathological features, were examined.
Methods: A total of 100 formalin-fixed paraffin-embedded urothelial carcinoma tissues
were selected from the Department of Pathology, Hospital Kuala Lumpur and the protein
expression of ISL1 and LHX5 was determined using immunohistochemistry.
Results: Positive expression of ISL1 and LHX5 was detected in 94% and 98% of the
samples, respectively. There were no distinct LHX5 expression patterns associated with different
cancer stages, but the proportion of high-expressing tumours was higher in high-grade tumours.
In addition, there was a significant association between the expression of LHX5 and tumour grade.
The proportion of tumours expressing high levels of ISL1 was found to be highest in later stage
tumours.
Conclusion: The high percentage of tumours expressing both these genes suggests that
ISL1 and LHX5 play an important role in bladder tumourigenesis across multiple stages.
- Full text:3.2020my0837.pdf
