Genetic analysis of mitochondrial pcox1 and ribosomal 18S rRNA genes in Eurytrema pancreaticum isolates from goats in Huaihua City, Hunan Province
10.16250/j.32.1374.2020126
- VernacularTitle:湖南省怀化地区山羊胰阔盘吸虫线粒体pcox1基因和核糖体18S rRNA 基因序列遗传变异研究
- Author:
Qiang-Hong HOU
1
;
Xiao-Huai ZHOU
2
;
Guo-Min YAO
1
;
Zhong-Bo LI
1
;
Ming SHU
1
;
Xiang WANG
1
;
Wei LUO
1
Author Information
1. Huaihua Vocational and Technical College, Hunan Province, Huaihua 418000, China
2. Xinhuang County Center of Animal Husbandry and Fishery Affairs, Hunan Province, China
- Publication Type:Journal Article
- Keywords:
Eurytrema pancreaticum;
Goat;
pcox1 gene;
18S rRNA gene;
Genetic variation;
Huaihua City
- From:
Chinese Journal of Schistosomiasis Control
2020;32(4):380-383
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genetic variation of Eurytrema pancreaticum isolated from goats in Huaihua City, Hunan Province. Methods The partial sequence of mitochondrial cytochrome I (pcox1) and ribosomal 18S rRNA genes were amplified using a PCR assay in E. pancreaticum isolates from goats in Huaihua City, Hunan Province, and the PCR amplification products were sequenced. Then, the gene sequences were subjected to genetic variation and phylogenetic analyses. Results The sequences of the pcox1 and 18S rRNA genes were 430 bp and 1 857 bp in length in 18 E. pancreaticum isolates from goats in Huaihua City, Hunan Province, and there were 14 and 35 variation sites in pcox1 and 18S rRNA gene sequences, with intra-species genetic variations of 0 to 1.4% and 0 to 0.8%, respectively. The sequences of pcox1 and 18S rRNA genes had 99.0% to 99.8% and 99.5% to 99.8% homologies with those from E. pancreaticum Chinese strain recorded in the GenBank database. Consistent phylogenetic analysis results were found based on pcox1 and 18S rRNA genes. The 18 E. pancreaticum isolates from goats in Huaihua City were clustered into a clade with the known E. pancreaticum isolates registered in GenBank, and the clade with these 18 E. pancreaticum isolates was close to the clades with Eurytrema species and far from the clades with other trematodes. Conclusions The E. pancreaticum isolates from goats have a low genetic variation in Huaihua City, Hunan Province. Mitochondrial pcox1 and ribosomal 18S rRNA genes may serve as molecular markers for the studies on the genetic variation in goat-derived E. pancreaticum.
