Establishment of UPLC-Q-TOF-MS/MS Fingerprint of Fritillaria thunbergii and Analysis of Anti-inflammatory Quality Markers
- VernacularTitle:浙贝母UPLC-Q-TOF-MS/MS指纹图谱的建立及其抗炎质量标志物的分析
- Author:
Bin CHENG
1
;
Aizhen ZHOU
1
;
Xin PENG
2
;
Xinlei WANG
3
;
Juan WANG
1
Author Information
1. School of TCM，Zhejiang Pharmaceutical College，Zhejiang Ningbo 315100，China
2. Ningbo Research Institute of Zhejiang University，Zhejiang Ningbo 315000，China
3. College of Pharmacy，Harbin University of Commerce，Harbin 150076，China
- Publication Type:Journal Article
- Keywords:
Fritillaria thunbergii;
Anti-inflammatory effect;
UPLC-Q-TOF-MS/MS;
Bivariate correlation analysis;
Grey
- From:
China Pharmacy
2020;31(17):2129-2135
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish UPLC-Q- TOF-MS/MS fingerprint of Fritillaria thunbergii ， and to define its anti-inflammatory quality markers. METHODS ：The determination was performed on Eclipse Plus C 18 column with mobile phase consisted of methanol- 0.1％ formic acid solution （gradient elution ）at the flow rate of 0.4 mL/min. The column temperature was set at 30 ℃，and injection volume was 2 µL. The electrospray ion source was used to scan in the range of m/z 50-1 200 with positive and negative ion detection mode. UPLC-Q-TOF-MS/MS fingerprints of 10 batches of F. thunbergii from different habitats were established by using the Similarity Evaluation System of TCM Chromatographic Fingerprints （2004A edition ）. With ear swelling degree，the serum levels of MDA and NO as anti-inflammatory indexes ，the correlation between the relative area of common peaks in fingerprint and the anti-inflammatory indexes was analyzed by using bivariate correlation analysis and grey correlation analysis ， and the quality markers were screened and identified. RESULTS ：In positive and negative ion mode ，10 batches of F. thunbergii had 26 peaks and 10 peaks. Based on bivariate correlation analysis and grey correlation analysis ，nine quality markers related to anti-inflammatory effect were found ，which were identified as peiminine ，peimine，cyclobalamine，daucidin，polyphyllin Ⅴ， bitumen podophyllotoxin ，phytosterol，ent-kaur-15-en-17-ol，ent-17-norkauran-16-one. CONCLUSIONS ：Established UPLC-Q- TOF-MS/MS fingerprint can be used to evaluate the quality of F. thunbergii ；peiminine，peimine and cyclobalamine and so on may be the quality markers of anti-inflammatory effect of F. thunbergii .
