Use of ITS2 and psbA-trnH DNA barcode sequences of wild Phytolacca resources in Guanzhong area of Shaanxi Province
10.16438/j.0513-4870.2020-0278
- VernacularTitle:陕西关中野生商陆资源的ITS2和psbA-trnH条形码序列研究
- Author:
Rui-hua LÜ
1
;
Zhao FENG
1
;
Tian-yi MA
1
;
Rui-hua LÜ
1
;
Ming-ying ZHANG
2
;
Liang PENG
2
;
Jing GAO
2
;
Gang ZHANG
2
Author Information
1. College of Medical Technology, Shaanxi University of Chinese Medicine, Xianyang 712046, China
2. College of Pharmacy and Shaanxi Qinling Application Development and Engineering Center of Chinese Herbal Medicine, Shaanxi University of Chinese Medicine, Xianyang 712046, China
- Publication Type:Research Article
- Keywords:
italic>Phytolacca acinosa Roxb.;
DNA barcode;
ITS2;
italic>psbA-trnH;
molecular identification
- From:
Acta Pharmaceutica Sinica
2020;55(8):1951-1956
- CountryChina
- Language:Chinese
-
Abstract:
In order to explore the use of DNA barcode in the identification of wild Phytolacca resources in the Shaanxi Guanzhong area, 29 DNA samples were amplified and sequenced by using the universal primers ITS2 and psbA-trnH. The sequences were spliced and proof-read by Codon CodeA aligner V3.0, followed by blast comparison and identification analysis; mega 6.0 was used to analyze sequence characteristics, Kimura 2-Parameter (K2P) was used to analyze distance and intraspecific or interspecific variation, and Neighbor-Joining trees were established to evaluate the ability of two pairs of candidate sequences to distinguish Phytolaccae Radix from its adulterants. The results showed that the success rate of PCR amplification and sequencing of ITS2 and psbA-trnH was 100%; the NJ tree showed that both ITS2 and psbA-trnH sequences could separate P. acinosa, P. americana, other species of the same genus like P. japonica, P. exiensis and two adulterant species into a single clade; primer ITS2 had an advantage over psbA-trnH in determining interspecific genetic distances. Therefore, both ITS2 and psbA-trnH sequences can be used for identification of Phytolacca and their adulterants, which provides a theoretical basis for the distribution of wild Phytolacca resources and their rational development and utilization.
