Quality control of red ginseng based on the ratio of ginsenosides Ro/Re
10.16438/j.0513-4870.2020-0132
- VernacularTitle:基于人参皂苷Ro/Re比例的红参质量标准研究
- Author:
Yuan-gui YANG
1
;
Ying-bo YANG
1
,
2
;
Zheng-cai JU
1
;
Yan-hai ZHANG
1
;
Li YANG
1
,
3
,
4
;
Zheng-tao WANG
1
,
3
,
4
Author Information
1. The MOE Key Laboratory of Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
2. Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China
3. Shanghai R&
4. D Center for Standardization of Chinese Medicines, Shanghai 201203, China
- Publication Type:Research Article
- Keywords:
red ginseng;
ginsenoside Ro;
quantitative analysis;
ginsenoside Ro/Re;
criterion revised
- From:
Acta Pharmaceutica Sinica
2020;55(8):1897-1902
- CountryChina
- Language:Chinese
-
Abstract:
Ginsenoside Ro decreased measures of inflammation, aging, oxidants and thrombus formation in a previous study. To measure ginsenoside Ro content in red ginseng from different years, an optimized extraction method was developed to determine ginsenoside Rg1, Re, Rb1 and Ro content by HPLC in 43 batches of red ginseng from different origins, growing years and manufacturers. The results indicate that the best extraction method was to ultrasonify a 1 g sample in 70% methanol for 50 min. The total running time of the optimized gradient was 50 min using a C18 core-shell column and was half the time described in the Chinese Pharmacopoeia, 2015 edition. The separation resolution of all of targeted compounds was greater than 1.6. The peak shape of ginsenoside Ro was optimal when the mobile phase consisted of acetonitrile and water with 0.1% phosphoric acid. The content of ginsenoside Ro was in the range of 0.11% to 0.43%, and the average content was 0.26%, which was higher than that of ginsenoside Rg1 and Re. The ratio of ginsenoside Ro and Re as a threshold could be used to discriminate red ginseng from different growing years; in addition, 100%, 94.4% and 46.6% of red ginseng from six, five and four years exceeded the threshold of 1.3. Our optimized analytical method for characterization of red ginseng is convenient and shortens the assay time.
