Simultaneous determination of multiple constituents of QingJinHuaTan Decoction by ultra high performance liquid chromatography
10.16438/j.0513-4870.2020-0010
- VernacularTitle:UHPLC法测定经典名方清金化痰汤物质基准中多指标成分的含量
- Author:
Jing LIU
1
;
Ran LIU
1
;
Dan-dan LI
1
;
Hua-rong XU
1
;
Qing LI
1
;
Kai-shun BI
1
Author Information
1. Shenyang Pharmaceutical University, Shenyang 110016, China
- Publication Type:Research Article
- Keywords:
classical prescription;
QingJinHuaTan Decoction;
UHPLC;
content determination;
quality control
- From:
Acta Pharmaceutica Sinica
2020;55(8):1872-1876
- CountryChina
- Language:Chinese
-
Abstract:
A UHPLC method for the simultaneous determination of multiple constituents in QingJinHuaTan Decoction was established. The separation was performed on a Waters cortecs T3 column (150 mm×2.1 mm, 1.6 μm); the mobile phase was acetonitrile-water (containing 0.04% phosphoric acid) with gradient elution at a flow rate of 0.30 mL·min-1, the column temperature at 25 ℃ and the wavelengths at 238 nm and 280 nm. The results showed that all peaks were well separated and all components had a good linear relationship in the investigative range, (r > 0.999). The repeatability and stability were good and the recovery was between 92.5%-104.7%. The method is simple, accurate and reliable and provides a basis for quality control of QingJinHuaTan Decoction and for further development of methods for its standardization.
