The inhibitory effect of calcium dobesilate on cisplatin induced HK-2 cell apoptosis and its mechanism
10.16438/j.0513-4870.2019-1068
- VernacularTitle:羟苯磺酸钙对顺铂诱导的HK-2细胞凋亡的抑制作用及其机制
- Author:
Chun-xia REN
1
;
Li-fei ZHU
1
;
Zi-cheng YU
1
Author Information
1. Department of Pharmacy, Yangpu Hospital, Tongji University, Shanghai 200090, China
- Publication Type:Research Article
- Keywords:
calcium dobesilate;
cisplatin;
human proximal tubular epithelial cell;
apoptosis;
oxidative stress
- From:
Acta Pharmaceutica Sinica
2020;55(8):1830-1835
- CountryChina
- Language:Chinese
-
Abstract:
We observed the effect of calcium dobesilate (CaD) on apoptosis induced by cisplatin in human proximal tubular epithelial cells (HK-2) and explored the possible mechanism. Based on HK-2 cells apoptosis model induced by cisplatin, CCK-8 method was used to detect the effect of CaD on the proliferation of HK-2. Apoptosis was detected by flow cytometry. Reactive oxygen species (ROS) assay was used to evaluate the level of oxidative stress. The mitochondrial membrane potential was measured by JC-1 method. The expression levels of p53, caspase-3, bcl-2 and bax in cisplatin-induced HK-2 were detected by Western blot. The expression of renal injury factor 1(KIM-1) and neutrophil gelatin-related apolipoprotein (NGAL), markers of acute kidney injury, were detected by ELISA. The results showed that CaD could reduce the oxidative stress level induced by cisplatin and inhibit apoptosis in renal tubular epithelial cells. Cisplatin can up-regulate the protein expressions of p53, caspase-3, bax, KIM-1 and NGAL, and reduce the expression of bcl-2. After using CaD, the protein levels of KIM-1, NGAL, p53, caspase-3 and bax were significantly reduced, while the levels of bcl-2 were increased. This study has shown that CaD can alleviate cisplatin-induced HK-2 injury and inhibit HK-2 apoptosis, which may be related to the regulation of bax/bcl-2/caspase-3 apoptosis signaling pathway.
