Effects of TGF-β1 on the migration of oral cancer-associated fibroblasts in two and three dimensional co-culture models
10.12016/j.issn.2096-1456.2020.09.003
- Author:
YANG Jin
1
,
2
,
3
;
WU Feifei
1
,
2
,
3
;
GAO Qinghong
4
;
LI Xiaoyu
1
,
2
,
5
;
MANABU Kato
6
;
CHENG Ran
1
,
2
,
7
;
ZHOU Hongmei
1
,
2
,
3
Author Information
1. State Key Laboratory of Oral Diseases &
2. National Clinical Research Center for Oral Diseases &
3. Department of Oral Medicine, West China Hospital of Stomatology, Sichuan University
4. Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University
5. West China Hospital of Stomatology, Sichuan University
6. Urology, Mie University Hospital
7. Department of Preventive Dentistry, West China Hospital of Stomatology, Sichuan University
- Publication Type:Journal Article
- Keywords:
carcinoma-associated fibroblasts;
migration;
three-dimensional cell culture model;
transforming growth factor-β1;
oral carcinoma
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2020;28(9):562-568
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of transforming growth factor-β1 (TGF-β1) on the migration of oral carcinoma associated fibroblasts (CAFs) with two-dimensional culture model and three-dimensional model.
Methods : Under two-dimensional culture conditions, CAFs stimulated by TGF-β1 with the addition of 10 ng/mL medium were used as the experimental group, and untreated CAFs were used as the control group. The migration of CAFs with the stimulation of TGF-β1 was measured by cell scratch assay and transwell assay. CAFs positive for green fluorescent protein (GFP) were cultured by retrovirus transfection. Human tongue squamous cell carcinoma cells SCC25, GFP(+) CAFs and CAFs with three-dimensional cell co-culture models were established. The three-dimensional model cultured under the stimulation of TGF-β1 with 10 ng/mL medium was used as the experimental group, and the three-dimensional model without TGF-β1 was used as the control group. The migration of CAFs with the stimulation of TGF-β1 was also measured by the three-dimensional models.
Results: It was verified that 10 ng/mL TGF-β1 promoted the migration of CAFs in the two-dimensional culture model. The three-dimensional co-culture models of SCC25, GFP(+) CAFs and CAFs were successfully established. The migration of SCC25 and CAFs was detected in the three-dimensional model. However, 10 ng/mL TGF-β1 had little effect on their migration.
Conclusion:The effect of TGF-β1 in vitro on the migration of oral CAFs was associated with different culture models in two and three dimensions.
- Full text:TGF-β1对口腔癌相关成纤维细胞在二维和三维共培养条件下迁移的影响.pdf
