Effect of penehyelidine hydrochloride on TIPE2-TLR4-MyD88 signaling pathway in a rat model of traumatic acute lung injury
10.3760∕cma.j.issn.0254-1416.2019.10.022
- VernacularTitle:盐酸戊乙奎醚对大鼠创伤性急性肺损伤时TIPE2-TLR4-MyD88信号通路的影响
- Author:
Weina DUAN
1
;
Min YUAN
;
Qian KONG
;
Yan LENG
;
Zhen QIU
;
Qin HUANG
;
Xiaojing WU
Author Information
1. 武汉大学人民医院麻醉科 430060
- Keywords:
Cholinergic antagonists;
Acute lung injury;
Ubiquitin-specific proteases;
Toll-like receptor 4;
Myeloid differentiation factor 88
- From:
Chinese Journal of Anesthesiology
2019;39(10):1237-1239
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of penehyelidine hydrochloride(PHCD)on tumor necrosis factor α-induced protein 8-like-2(TIPE2)-Toll-like receptor 4(TLR4)-myeloid differentiation fac-tor 88(MyD88)signaling pathway in a rat model of traumatic acute lung injury(ALI).Methods Thirty SPF healthy male Sprague-Dawley rats,aged 8 weeks,weighing 190-210 g,were divided into 3 groups(n＝15 each)by a random number table method: sham operation group(group Sham),traumatic ALI group(group ALI)and group PHCD.ALI was induced by blunt chest trauma in ALI and PHCD groups.PHCD 2 mg/kg was intraperitoneally injected immediately after blunt chest trauma in group PHCD.The rats were sacrificed and lung tissues were removed at 8 h after the model was successfully established for exami-nation of the pathological changes and ultrastructure of lung tissues(with a light microscope or an electron microscope)and for determination of the wet to dry weight ratio(W/D ratio)and expression of TLR4 and MyD88 in lung tissues.Results Compared with group Sham,the W/D ratio was significantly increased,TIPE2 expression was down-regulated,and the expression of TLR4 and MyD88 was up-regulated in ALI and PHCD groups(P＜0.05).Compared with group ALI,the W/D ratio was significantly decreased,TIPE2 expression was up-regulated,and the expression of TLR4 and MyD88 was down-regulated(P＜0.05),and the pathological changes of lung tissues and ultrastructure were significantly attenuated in group PHCD.Conclusion The mechanism by which PHCD reduces traumatic AIL is related to activating TIPE2-TLR4-MyD88 signaling pathway in rats.
