Effects of different hemoglobin concentrations on lung injury in a rabbit model of hemorrhagic shock and resuscitation
10.3760∕cma.j.issn.0254-1416.2019.10.021
- VernacularTitle:不同血红蛋白浓度对失血性休克复苏家兔肺损伤的影响
- Author:
Xiaoqian LIU
1
;
Li LIU
;
Xun ZHOU
;
Nana ZHU
;
Yongquan CHEN
;
Xiaoju JIN
;
Jianrong GUO
Author Information
1. 海军军医大学附属公利医院麻醉科
- Keywords:
Shock,hemorrhagic;
Cardiopulmonary resuscitation;
Hemoglobins;
Acute lung injury
- From:
Chinese Journal of Anesthesiology
2019;39(10):1233-1236
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of different hemoglobin(Hb)concentrations on the lung injury in a rabbit model of hemorrhagic shock and resuscitation(HS-R).Methods Fifty healthy male New Zealand rabbits,aged 2 months,weighing 1.9-2.4 kg,were divided into 5 groups(n＝10 each)u-sing a random number table method: control group(group C),HS-R group(group H),and three HS-R plus infusion of concentrated red blood cell groups group R1(60 g/L≤Hb＜80 g/L),group R2(80 g/L≤Hb＜100 g/L)and group R3(100 g/L≤Hb＜120 g/L).The animals were sacrificed at 3 h after resuscita-tion,lung tissues were obtained for examination of the pathological changes with a light microscope,and lung tissues were obtained again for determination of wet to dry weight ratio(W/D ratio),neutrophil my-eloperoxidase(MPO),NO level and cell apoptosis(by TUNEL).Results Compared with group C,the levels of MAP and NO,W/D ratio and apoptosis index were significantly increased in the other groups(P＜0.05).Compared with group H,the levels of MAP and NO,W/D ratio and apoptosis index were signifi-cantly decreased in R2 and R3 groups(P＜0.05).Compared with group R2,the apoptosis index was signif-icantly increased at T5(P＜0.05),and no significant change was found in the other parameters in group R3(P>0.05).Conclusion Maintaining Hb 80-100 g/L after HS-R reduces acute lung injury in rabbits.
