Effect of NADPH on myocardial ischemia-reperfusion injury in rats
10.3760∕cma.j.issn.0254-1416.2019.10.010
- VernacularTitle:NADPH对大鼠心肌缺血再灌注损伤的影响
- Author:
Yifei WANG
1
;
Jiang ZHU
;
Peimin CHEN
;
Liuhui CHANG
;
Hong XIE
Author Information
1. 苏州大学附属第二医院麻醉科 215000
- Keywords:
NADPH;
Myocardial reperfusion injury
- From:
Chinese Journal of Anesthesiology
2019;39(10):1185-1188
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of nicotinamide adenine dinucleotide phosphate(NADPH)on myocardial ischemia-reperfusion(I/R)injury in rats.Methods Fifty-six SPF adult male Sprague-Dawley rats,weighing 220-320 g,aged 1-2 months,were divided into 4 groups(n＝14 each)using a random number table method: sham operation group(Sham group),myocardial I/R group(I/R group),NADPH group(N group)and diltiazem group(D group).The model of myocardial I/R injury was established by ligation of the left anterior descending branch for 30 min followed by 2-h reperfusion in anesthetized rats.NADPH 16 mg/kg was intravenously infused over 5 min starting from 5 min of reperfusion in N group.Diltiazem 5 mg/kg was infused through the internal jugular vein starting from 10 min before is-chemia until the end of ischemia.At 2 h of reperfusion,blood samples were taken from the internal jugular vein for measurement of serum LDH and cTnI concentrations,and myocardial tissues were taken for deter-mination of infarct size and ROS level.Results Compared with Sham group,the serum LDH and cTnI concentrations,myocardial infarction size and ROS levels were significantly increased in I/R group(P＜0.05).Compared with I/R group,the serum LDH and cTnI concentrations,myocardial infarction size and ROS levels were significantly decreased in N and D groups(P＜0.05).Compared with N group,the LDH concentration was significantly decreased(P＜0.05),and no significant change was found in the cTnI con-centration,myocardial infarction size or ROS level in D group(P>0.05).Conclusion NADPH can re-duce myocardial I/R injury through antioxidant effect in rats.
