Methanolic extract of Clausena excavata promotes wound healing via anti-inflammatory and anti-apoptotic activities
10.4103/2221-1691.281467
- Author:
Shaymaa Fadhel Abbas Albaayit
1
;
Rasedee ABDULLAH
;
Abdullah NOORLIDAH
;
Abba YUSUF
Author Information
1. Department of Biology
- Keywords:
Clausena excavata leaves;
Anti-inflammatory cytokines;
Cytotoxicity;
Apoptotic genes
- From:Asian Pacific Journal of Tropical Biomedicine
2020;10(5):232-238
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the anti-inflammatory properties of methanolic extract of Clausena excavata in lipopolysaccharide (LPS)-activated macrophages (J774A.1) and the effect on skin wound in a rat model through determining cytokine levels and gene expressions.Methods: The effects of methanolic extract of Clausena excavata on in vitro viability and TNF-α, IL-6, IL-10, and nitric oxide release by LPS-activated J774A.1 cells were determined. In addition, relative expressions of BAX, BCL-2 and COX-2 genes were examined in healed wounds of rats. Results: The methanolic extract of Clausena excavata was not toxic to J774A.1 cells at the highest dose of 400 μg/mL. It decreased levels of TNF-α and IL-6, while increasing IL-10 level in LPS-activated J774A.1 cells and in the healed wounds of rats. The methanolic extract of Clausena excavata also inhibited nitric oxide production in LPS-activated J774A.1 cells. The BAX and COX-2 genes were downregulated while the BCL-2 gene was upregulated in the healed wound of rats. Conclusions: The methanolic extract of Clausena excavata promotes wound healing via its anti-inflammatory and anti-apoptotic activities.
