Inhibitory effect of PARP inhibitor olaparib on acute myeloid leukemia HL-60 cells
10. 11904/j. issn. 1002-3070. 2019. 06. 001
- VernacularTitle:PARP抑制剂olaparib对急性髓系白血病细胞HL-60抑制作用研究
- Author:
Zhichao ZHU
1
;
Yu BAI
;
Xuzhang LU
;
Xiao SUN
;
Liuyang HE
;
Chunjian QI
Author Information
1. 南京医科大学附属常州市第二人民医院中心实验室(常州 213000)
- Keywords:
Olaparib;
PARP inhibitor;
HL-60 cells;
Acute myeloid leukemia
- From:Practical Oncology Journal
2019;33(6):481-485
- CountryChina
- Language:Chinese
-
Abstract:
Objective The inhibitory effect of the PARP inhibitor olaparib on human acute myeloid leukemia HL-60 cells was studied. Methods The HL-60 cells in logarithmic growth phase were treated with different concentrations(1. 25,2. 5,5 and 10 μmol/L) of olaparib for different time. The CCK-8 assay was used to detect the inhibitory effect of olaparib on HL-60 cells. The apoptotic level of HL-60 cells was detected by Annexin-V/PI double staining method,and the expression of related signal proteins ( PARP-1 and caspase-3)in HL-60 cells was detected by Western blot. Results HL-60 cells were inhibited by olaparib at dif-ferent concentrations(1. 25,2. 5,5 and 10 μmol/L) for 48 h,and the inhibition rate gradually increased with the prolongation of the action time;at the same time,the apoptotic rate was increased in HL-60 cells after olaparib treatment for 48 h,showing a dose-de-pendent manner;the PARP activity was inhibited and caspase-3 was activated in HL-60 cells treated with olaparib. Conclusion The PARP inhibitor olaparib not only inhibits proliferation of HL-60 cells,but it also promotes apoptosis of HL-60 cells by inhibi-ting PARP activity and activating caspase-3.
