Establishment of experimental model of tracheomalacia in beagle dogs
- VernacularTitle:比格犬气管软化模型建立
- Author:
Wenhao LIU
1
,
2
;
Kaifu ZHENG
1
,
2
;
Lei WANG
1
,
2
;
Hao ZHANG
1
,
2
;
Jinjie JI
2
,
3
;
Xiaofei LI
1
,
2
;
Jinbo ZHAO
1
,
2
Author Information
1. Department of Thoracic Surgery, Tangdu Hospital, Air Force Medical University, Xi&rsquo
2. an, 710032, P.R.China
3. Department of Anesthesiology, Tangdu Hospital, Air Force Medical University, Xi&rsquo
- Publication Type:Journal Article
- Keywords:
Tracheomalacia;
animal model;
trachea cartilage removal
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2020;27(07):824-829
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a model of tracheomalacia in beagle dogs. Methods Six healthy male beagles were selected with a weight of 12-15 kg and age of 12-18 months. The dog was placed in supine position after being anesthetized. Then midline incision was performed on dogs' cervical skin and main trachea was dissected. Six continuous cartilage rings separated from the tracheal wall were removed. Finally, the endotracheal mucosal was examined and the wound was sutured layer by layer. Different degrees of cartilage were removed to simulate different degrees of tracheomalacia. The beagle dogs were classified into two groups (n=3 in each group): a mild tracheomalacia (MTM) group (part of the cartilage near the trachea membrane was retained) and a severe tracheomalacia (STM) group (cartilage was removed as much as possible). Results The dogs in the MTM group survived for a long time after the operation, showing symptoms of airway stenosis such as wheezing and coughing. The dogs were killed at postoperative week 2, and the pathological examination was performed. In the STM group, severe asphyxia occurred in the experimental animals after tracheal intubation removed, and all dogs died within 1 hour after surgery. Postoperative bronchoscopy revealed that the trachea of the MTM group dogs collapsed in the phase of inhalation, but it could maintain a certain patency. The trachea of the STM group dogs collapsed completely in the phase of inhalation. Postoperative X-ray showed that the diameter of the airway in the MTM group was reduced and trachea did not completely collapse. In the STM group, the trachea collapsed completely at the cartilage removed segment. Pathological examination showed that the cartilage in the MTM group was partially removed and tracheomalacia was obvious in the cartilage removed segment. In the STM group, most of the cartilage was removed with only few cartilages left. Conclusion The clinical symptoms of tracheomalacia in different degrees can be simulated and repeatable. Animal models can be established by controlling the degree of removal of tracheal cartilage ring in dogs. This method provides a simple, repeatable and standardized large animal model for the treatment and transformation of tracheomalacia.
