Intervention Effects of Berberine on Mice Macrophage Polarization Based on TLR 4/MyD88/NF-κB Signaling Pathway
- VernacularTitle:基于TLR4/MyD88/NF-κB信号通路研究黄连素对小鼠巨噬细胞极化的干预作用
- Author:
Jiangong LI
1
;
Wenxi SUN
2
;
Jiayue LIU
3
;
Xueshan LI
1
;
Weiqi XUE
4
;
Chuanjin LUO
4
Author Information
1. Dept. One of Internal Medicine，Zhongshan Hospital of TCM，Guangdong Zhongshan 528400，China
2. Clinical College of Acupuncture and Rehabilitation，Guangzhou University of TCM，Guangzhou 510405，China
3. The First Clinical Medical College，Guangzhou University of TCM，Guangzhou 510405，China
4. Dept. of Cardiovascular Disease，the First Affiliated Hospital of Guangzhou University of TCM，Guangzhou 510405，China
- Publication Type:Journal Article
- Keywords:
Berberine;
Mice macrophage;
RAW264.7 cell;
Polarization;
TLR4/MyD88/NF-κB signaling pathway
- From:
China Pharmacy
2020;31(15):1804-1809
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study the effects of berberine on mic e macrophage polarization based on TLR 4-MyD88-NF-κB signaling pathway. METHODS ：Using mice RAW 264.7 macrophage as the object ，atorvastatin calcium as positive control ， inflammatory cell model was induced by lipopolysaccharide （LPS）；ELISA method was used to detect the contents of TNF-α，IL-6 and NF-κB in cell culture medium after treated with low，medium and high doses of berberine （5，10，20 μmol/L）for 24 h. The real-time fluorescence quantitative PCR was conducted to determine the mRNA expression of TLR 4 and MyD 88 in cells. Western blotting assay was used to detect the protein expression of TLR 4，MyD88，iNOS and CD 206 in cells. RESULTS ：Compared with blank control group ，the contents of TNF-α，IL-6 and NF-κB in cell culture medium，mRNA expression of TLR 4 and MyD 88， protein expression of TLR 4，MyD88 and iNOS in cells were increased significantly in LPS induction group （P＜0.05）. Compared with LPS induction group ，the contents of TNF-α and IL-6，mRNA and protein expression of TLR 4 and MyD 88 in atorvastatin calcium group ，berberine medium-dose and high-dose groupsas well as the content of NF-κ B and protein expression of iNOS in administration groups were decreased significantly ， while the content of NF-κB in berberine high-dose group was significantly lower than atorvastatin calcium group （P＜0.05）. The protein expressions of CD206 in atorvastatin calcium group and berberine high-dose group were increased significantly ，while the protein expression of CD 206 in berberine high-dose group was significantly higher than atorvastatin calcium group （P＜0.05）. CONCLUSIONS ：Different doses of berberine can intervene in mice macrophage polarization to different extents ，the mechanism of which may be associated with the regulation of TLR4/MyD88/NF-κB signaling pathway.
