Effects of high glucose on osteogenic differentiation of hBMSC
10.12016/j.issn.2096-1456.2017.01.005
- Author:
GAO Hai
1
,
2
;
CHEN Xiao
1
,
2
;
GUAN Dong-hua
1
,
2
;
ZHANG Ying-jie
2
,
3
;
LIN Chang-lü
1
,
2
Author Information
1. Department of Prosthodontics, Stomatological Hospital of Southern Medical Unversity &
2. Guangdong Provincial Stomatological Hospital
3. Department of Orthodontics, Stomatological Hospital of Southern Medical Unversity &
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus;
Osteointegration;
Dental implants;
Osteogenic differentiation;
hBMSC
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2017;25(1):26-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of different glucose concentration on the proliferation and osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSC) in vivo
Methods:Cultured with basal medium containing different glucose concentrations, CCK-8 cell proliferation was detected at 1, 4, 7, 10 days. The osteogenic differentiation of human bone marrow mesenchymal stem cells was observed at 7 d, which was induced by osteogenic differentiation medium with different concentration of glucose. The expressions of alkaline phosphatase (ALP), osteocalcin (OC) and collagen type I (Col-1) gene were detected by real-time fluorescence quantitative PCR. Mineralized nodule formation was displayed by calciumalizarin red staining on the seventh day.
Results :10 mM glucose stimulated proliferation of hBMSC, while the higher (>30 mM) inhibited the proliferation (P < 0.05); Osteogenic induction can induce osteogenic differentiation of hBMSC, but the increase of glucose concentration will decrease the formation of mineralized nodules of hBMSC, inhibit the expression of osteogenic marker genes ALP, OC and Col-1 (P < 0.05).
Conclusion:The expression of Col-1, ALP and OC in osteoblast was down-regulated by high glucose, and the hBMSC proliferation was inhibited. At the same time, high glucose can reduce the osteogenic mineralization ability of stem cells and indirectly affect bone formation and metabolism.
- Full text:高糖对人骨髓间充质干细胞成骨分化的影响.pdf
