Effects of arecoline and calcium ion on the permeability of dysplastic oral epithelia model in vitro
10.12016/j.issn.2096-1456.2017.01.004
- Author:
HOU Dong-lan
1
;
CHEN Rong
1
;
GAO Yi-jun
1
;
YIN Xiao-min
2
Author Information
1. Oral Medical Center of the Second Xiangya Hospital of Central South University
2. Center of Stomatology of Xiangya hospital, Central South University
- Publication Type:Journal Article
- Keywords:
Arecoline;
Dysplastic oral epithelia;
In vitro model;
Calcium;
Permeability
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2017;25(1):21-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To study the effects of arecolineand Calcium ion (Ca 2+) on the permeability of dysplastic oral epithelia model in vitro.
Methods:To establish the dysplastic oral epithelia model in vitro by culturing oral keratinocyte(DOK) and harvesting a DOK cell monolayer. The models were divided into control group, arecoline group and "Ca 2++ arecoline" group. The values of Lucifer Yellow Papp were used to estimate the permeability changes of models after treatedwith arecoline and Ca 2+.
Results : In arecoline group, the lucifer yellow Papp values of each subgroup were higher than that of control group (P < 0.05), and the values increased as arecoline concentration elevated and time lasted (P < 0.05). When the "Ca 2++ arecoline" group were pretreated with Ca 2+, the values of subgroup "10 μg/mL" was lower than that of the corresponding arecoline group (P > 0.05). However, the value of subgroup "4 h 10 μg/mL" of "Ca 2++ arecoline" group had no statistical difference with that of control group (P > 0.05), while the other subgroups were increased (P < 0.05); Besides, these values in "Ca 2++ arecoline" group were increased as the arecoline concentration elevated and time lasted too (P < 0.05).
Conclusion:Intervention of arecoline contributes to the increase of permeability of DOK cell monolayer model, which maybe an important reason for the cancerization of dysplastic oral epithelia, however Ca 2+might weaken these effects of arecoline in the process.
- Full text:槟榔碱和Ca 2 对口腔黏膜异常增生上皮体外模型通透性的影响.pdf
