Fuzheng Yanggan Fomula alleviates liver injury through inhibiting NLRP3 inflammasome
10.16438/j.0513-4870.2020-1004
- VernacularTitle:扶正养肝合剂抑制NLRP3炎症小体活化缓解肝损伤作用
- Author:
Xi WANG
1
,
2
,
3
;
Xiang LUO
1
,
2
,
3
;
Lei LIANG
1
,
2
,
3
;
Jin ZHAO
4
;
Yan-hui SUN
5
;
Hong-shuai XU
5
;
Xi-qiang CAI
5
;
Hiroshi KURIHARA
1
,
2
,
3
;
Yi-fang LI
1
,
2
,
3
;
Rong-rong HE
1
,
2
,
3
Author Information
1. Guangdong Engineering Research Center of Chinese Medicine and Disease Susceptibility, Jinan University, Guangzhou 510632, China
2. Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, Jinan University, Guangzhou 510632, China
3. Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China
4. Jinzhou Central Hospital, Jinzhou 121017, China
5. Jinzhou Infectious Disease Hospital, Jinzhou 121017, China
- Publication Type:Research Article
- Keywords:
Nod-like receptor protein 3 inflammasome;
Fuzheng Yanggan Fomula;
italic>Propionibacterium acnes/lipopolysaccharide;
acute liver injury;
interleukin-1β
- From:
Acta Pharmaceutica Sinica
2020;55(7):1627-1633
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the model of Propionibacterium acnes/lipopolysaccharide (P. acnes/LPS)-induced acute liver injury in mice was employed to investigate the protective effects of Fuzheng Yanggan Fomula (FYF) on acute liver injury. The effects of FYF on the contents of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and interleukin-1β (IL-1β) in the serum, and the levels of malondialdehyde (MDA), oxygen radical absorbance capacity (ORAC), and glutathione (GSH) were examined in the livers of mice treated with P. acnes/LPS; The protein expression levels of Nod-like receptor protein 3 (NLRP3), apoptosis-associated speck-like protein containing a CARD (ASC), cysteinyl aspartate specific proteinase-1 (caspase-1), and IL-1β in liver tissues were detected by Western blot; Furthermore, hematoxylinendash-eosin (HE) staining, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, and immunohistochemical assay were used to observe pathological changes, apoptosis index, and inflammation infiltration of the liver tissue sections. All animal welfare and experimental procedures were followed by the Animal Ethics Committee of Jinan University. We conclude that FYF could alleviate P. acnes/LPS induced pathological damage and inflammatory infiltration in the liver of mice. Meanwhile, FYF decreases the contents of ALT, AST, IL-1β, and MDA, increases the contents of ORAC and GSH, and downregulates the expression of caspase-1 and IL-1β proteins. Collectively, these findings suggested that FYF could alleviate P. acnes/LPS induced acute liver injury in mice by inhibiting the activation of NLRP3 inflammasome, which provides a theoretical basis and a new drug target for the prevention and treatment of liver injury.
