Effect of parthenolide on proliferation and migration of MCF-7 breast cancer cells by targeting the c-myc G-quadruplex
- VernacularTitle:小白菊内酯通过靶向c-myc G-四链体抑制乳腺癌细胞增殖和迁移
- Author:
Yu-qing WANG
1
;
Yue GAO
1
;
Rong WEI
1
;
Rang LI
1
;
Pei-min HUANG
1
;
Chun-rong HUANG
1
;
Chao ZHANG
1
;
Yi-wen TAO
1
;
Jian-ye ZHANG
1
Author Information
- Publication Type:Research Article
- Keywords: parthenolide; G-quadruplex; breast cancer; proliferation; migration
- From: Acta Pharmaceutica Sinica 2020;55(7):1622-1626
- CountryChina
- Language:Chinese
-
Abstract:
This research investigated the effect of parthenolide on the proliferation and migration of human breast cancer cells and explored the molecular mechanism of that effect
. Surface plasmon resonance and fluorescence resonance energy transfer melting were used to detect the binding and stabilizing ability of PTL and G-quadruplex. MTT assays were used to determine the effect of PTL on the proliferation of MCF-7 breast cancer cells. A wound healing assay was performed to detect the migration of MCF-7. The results indicate that PTL shows good binding and stabilizing activities with c-myc G-quadruplex with aK D = 13.1 μmol·L-1. PTL inhibited the proliferation of MCF-7 cells with an IC50 of 21.3 μmol·L-1 (24 h), 14.5 μmol·L-1 (48 h) and 9.1 μmol·L-1 (72 h). PTL inhibited MCF-7 breast cancer cell proliferation and migration and down-regulated the transcription and expression level of c-myc by targeting G-quadruplex.
